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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

In this review, recent advances in the combination of CRISPR–Cas systems with graphene-based electrolyte-gated transistors are discussed in detail. In the first part, the functioning of CRISPR–Cas systems is briefly explained, as well as the most common ways to convert their molecular activity into measurable signals. Other than optical means, conventional electrochemical transducers are also developed. However, it seems that the incorporation of CRISPR/Cas systems into transistor devices could be extremely powerful, as the former provides molecular amplification, while the latter provides electrical amplification; combined, the two could help to advance in terms of sensitivity and compete with conventional PCR assays. Today, organic transistors suffer from poor stability in biological media, whereas graphene materials perform better by being extremely sensitive to their chemical environment and being stable. The need for fast and inexpensive sensors to detect viral RNA arose on the occasion of the COVID-19 crisis, but many other RNA viruses are of interest, such as dengue, hepatitis C, hepatitis E, West Nile fever, Ebola, and polio, for which detection means are needed.

Details

Title
CRISPR–Cas Systems Associated with Electrolyte-Gated Graphene-Based Transistors: How They Work and How to Combine Them
Author
Guermonprez, Pierre 1 ; Nioche, Pierre 2   VIAFID ORCID Logo  ; Renaud, Louis 3 ; Battaglini, Nicolas 1   VIAFID ORCID Logo  ; Sanaur, Sébastien 4   VIAFID ORCID Logo  ; Krejci, Eric 5   VIAFID ORCID Logo  ; Piro, Benoît 1   VIAFID ORCID Logo 

 ITODYS, CNRS, Université Paris Cité, F-75006 Paris, France; [email protected] (P.G.); [email protected] (N.B.) 
 INSERM US 36|CNRS UAR 2009, Structural and Molecular Analysis Platform, Université Paris Cité, F-75006 Paris, France; [email protected]; INSERM U1124, Université Paris Cité, F-75006 Paris, France 
 Institut des Nanotechnologies de Lyon INL-UMR5270, Université Lyon 1, F-69622 Villeurbanne, France; [email protected] 
 Department of Flexible Electronics, Institut Mines-Telecom, Mines Saint-Étienne, F-13541 Gardanne, France; [email protected] 
 CNRS, ENS Paris Saclay, Centre Borelli UMR 9010, Université Paris Cité, F-75006 Paris, France; [email protected] 
First page
541
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
20796374
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3132880425
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.