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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Background/Objectives: Osteoarthritis (OA) is the most common joint disease in the adult population. OA is the result of multiple mechanisms leading to inflammation and the degradation of the cartilage. A complex series of etiological actors have been identified so far, including extracellular vesicles (EVs). The EV content of the synovial fluid (SF) can release inflammatory mediators that enhance OA progression. An intra-articular viscosupplementation of high-MW hyaluronic acid (HyA) constitutes the first-line conservative treatment for OA. Although attractive for the potential pharmacological implications, the possibility that HyA may interact with EVs in the context of OA has not yet been specifically investigated; therefore, the present study aimed to fill this gap. Methods: We studied the effect of a HyA preparation (a blend of crosslinked and linear polymers, CLHyA) on the relevant inflammatory markers in chondrocytes (HC cells or primary chondrocytes isolated from patients with advanced OA) exposed to the EVs collected from IL-1β-stimulated THP-1 human monocytes (EVs+). Results: EVs+ caused specific inflammatory responses in chondrocytes that could be prevented by coincubation with CLHyA. This anti-inflammatory activity is likely dependent on the direct binding of CLHyA to CD44 receptors highly expressed in EVs+ and on the subsequent hindrance to EVs+ diffusion and docking to target cells. Conclusions: On the whole, the tight interactions identified herein between HMW HyA and EVs+ represent a novel, pharmacologically exploitable mechanism potentially relevant in the context of OA treatment.

Details

Title
Hyaluronic Acid Hampers the Inflammatory Response Elicited by Extracellular Vesicles from Activated Monocytes in Human Chondrocytes
Author
Carrabs, Vittoria 1 ; Guillén, Maria Isabel 2 ; Ferrándiz, María Luisa 3   VIAFID ORCID Logo  ; Alcaraz, María José 3   VIAFID ORCID Logo  ; Ferrini, Fabio 4   VIAFID ORCID Logo  ; Agostini, Rachele 4   VIAFID ORCID Logo  ; Guescini, Michele 4   VIAFID ORCID Logo  ; Fimognari, Carmela 5   VIAFID ORCID Logo  ; Capparucci, Italo 4 ; Barbieri, Elena 4   VIAFID ORCID Logo  ; Sestili, Piero 4   VIAFID ORCID Logo 

 Dipartimento di Scienze Biomolecolari, University of Urbino Carlo Bo, 61029 Urbino, Italy; [email protected] (V.C.); [email protected] (R.A.); [email protected] (M.G.); [email protected] (I.C.); [email protected] (E.B.); Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, 46010 Valencia, Spain; [email protected] (M.I.G.); [email protected] (M.L.F.); [email protected] (M.J.A.); Departamento de Farmacia, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, 46115 Valencia, Spain 
 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, 46010 Valencia, Spain; [email protected] (M.I.G.); [email protected] (M.L.F.); [email protected] (M.J.A.); Departamento de Farmacia, Facultad de Ciencias de la Salud, Universidad CEU Cardenal Herrera, 46115 Valencia, Spain 
 Instituto Interuniversitario de Investigación de Reconocimiento Molecular y Desarrollo Tecnológico (IDM), Universitat Politècnica de València, Universitat de València, 46010 Valencia, Spain; [email protected] (M.I.G.); [email protected] (M.L.F.); [email protected] (M.J.A.) 
 Dipartimento di Scienze Biomolecolari, University of Urbino Carlo Bo, 61029 Urbino, Italy; [email protected] (V.C.); [email protected] (R.A.); [email protected] (M.G.); [email protected] (I.C.); [email protected] (E.B.) 
 Dipartimento di Scienze per la Qualità della Vita, Università degli Studi di Bologna, C.so d’Augusto 237, 47921 Rimini, Italy; [email protected] 
First page
1386
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
19994923
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3133274730
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.