It appears you don't have support to open PDFs in this web browser. To view this file, Open with your PDF reader
Abstract
This study introduces a low-voltage electroporation microchip designed for transfection in cat embryos, featuring real-time impedance monitoring. The microchip uses a field constriction strategy, which localises the electric field to the membrane region in contact with the micro-orifice, enhancing electroporation efficiency while minimising damage. Embryos were positioned on the orifice, and a series of voltage pulses (10, 15, and 20 V) were applied. Electroporation efficacy was assessed using fluorescent dyes, followed by real-time impedance measurements to monitor the membrane resealing time. It provided valuable insights into membrane recovery times, essential for optimizing gene editing conditions to ensure efficient delivery and maintain cell integrity. The results demonstrated that the microchip with 15 V achieved a 69.5% higher electroporation rate and 100% of survival compared to conventional devices (p < 0.05). Additionally, the microchip successfully facilitated the transfer of green fluorescent protein genes into embryos, achieving a 78.5% success rate significantly greater compared to 53.6% with the conventional device (p < 0.05). This innovative microchip provides transformative transfection technology for safer and more efficient genomic modifications in embryos. It holds promising applications across species and therapeutic interventions, paving the way for future studies in advanced genomic research.
You have requested "on-the-fly" machine translation of selected content from our databases. This functionality is provided solely for your convenience and is in no way intended to replace human translation. Show full disclaimer
Neither ProQuest nor its licensors make any representations or warranties with respect to the translations. The translations are automatically generated "AS IS" and "AS AVAILABLE" and are not retained in our systems. PROQUEST AND ITS LICENSORS SPECIFICALLY DISCLAIM ANY AND ALL EXPRESS OR IMPLIED WARRANTIES, INCLUDING WITHOUT LIMITATION, ANY WARRANTIES FOR AVAILABILITY, ACCURACY, TIMELINESS, COMPLETENESS, NON-INFRINGMENT, MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE. Your use of the translations is subject to all use restrictions contained in your Electronic Products License Agreement and by using the translation functionality you agree to forgo any and all claims against ProQuest or its licensors for your use of the translation functionality and any output derived there from. Hide full disclaimer
Details
1 Chulalongkorn University, Department of Obstetrics, Gynaecology, and Reproduction, Faculty of Veterinary Science, Bangkok, Thailand (GRID:grid.7922.e) (ISNI:0000 0001 0244 7875); Chulalongkorn University, Center of Excellence for Veterinary Clinical Stem Cells and Bioengineering, Bangkok, Thailand (GRID:grid.7922.e) (ISNI:0000 0001 0244 7875)
2 Chulalongkorn University, Department of Electrical Engineering, Faculty of Engineering, Bangkok, Thailand (GRID:grid.7922.e) (ISNI:0000 0001 0244 7875); Chulalongkorn University, Micro/Nano-Electro-Mechanical Integrated System Research Unit, Faculty of Engineering, Bangkok, Thailand (GRID:grid.7922.e) (ISNI:0000 0001 0244 7875)
3 Rajamangala University of Technology Thanyaburi, Department of Electrical Engineering, Faculty of Engineering, Pathum Thani, Thailand (GRID:grid.440403.7) (ISNI:0000 0004 0646 5810)
4 National Science and Technology Development Agency (NSTDA), Thai Microelectronics Center (TMEC), Chachoengsao, Thailand (GRID:grid.425537.2) (ISNI:0000 0001 2191 4408)
5 Kyungpook National University, College of Veterinary Medicine, Daegu, Republic of Korea (GRID:grid.258803.4) (ISNI:0000 0001 0661 1556); nSAGE Inc., Incheon, Republic of Korea (GRID:grid.258803.4)
6 Seoul National University, Department of Veterinary Clinical Science, College of Veterinary Medicine and the Research Institute of Veterinary Science, Seoul, Republic of Korea (GRID:grid.31501.36) (ISNI:0000 0004 0470 5905)