Correction: Gao et al. High Expression of PDK4 Could Play a Potentially Protective Role by Attenuating Oxidative Stress after Subarachnoid Hemorrhage. J. Clin. Med. 2022, 11, 3974

Abstract

The fluorescent pictures of the SAH 2d group in Figure 3 and bands of the PDK4 and PDH groups in Figure 4 were wrongly chosen. MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. (B–D) Quantitative analysis of Western blot results showed that the ratio of p-ASK1/ASK1, p-P38/P38 and cleaved-caspase3/caspase3 were significantly increased after SAH. (B) Quantitative analysis of Western blot results showed that the ratio of p-PDH/PDH was significantly increased after Hb stimulation.

Error in Figure

In the original publication [1], there were mistakes in Figures 3 and 4 as published. The fluorescent pictures of the SAH 2d group in Figure 3 and bands of the PDK4 and PDH groups in Figure 4 were wrongly chosen. The corrected figures appear below.

In Figure 3, the fluorescent pictures of the SAH 2d group have been corrected.

In Figure 4, the bands of the PDK4 and PDH groups have been corrected.

Correct Email

Xun-Zhi Liu’s email address was changed from “[email protected]” to “[email protected]”.

The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.

Footnotes

Disclaimer/Publisher’s Note: The statements, opinions and data contained in all publications are solely those of the individual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content.

Figures

Figure 3. The apoptosis pathway was activated after SAH. (A) Representative bands of ASK1, p-ASK1, P38, p-P38, caspase3, cleaved-caspase3, Bax, and Bcl-2 expression in the cortex at each time point after SAH. (B–D) Quantitative analysis of Western blot results showed that the ratio of p-ASK1/ASK1, p-P38/P38 and cleaved-caspase3/caspase3 were significantly increased after SAH. (E) Quantitative analysis of Western blot and qPCR results showed that the protein and mRNA levels of Bax and Bcl-2 were increased after SAH. (F) Representative TUNEL staining in the cortex of right temporal lobe after SAH (TUNEL, green; NeuN, red; DAPI, blue). Bars represent the means ± SD. * p [less than] 0.05; ** p [less than] 0.01; vs. sham (n = 5 in each group). Bar = 50 μm.

Figure 4. The expression of PDKs in cultured primary neurons after Hb stimulation. (A) Representative bands of PDK1, PDK2, PDK3, PDK4, PDH, and p-PDH expression at each time point (0, 1, 3, 6, 12, and 24 h) after Hb stimulation. (B) Quantitative analysis of Western blot results showed that the ratio of p-PDH/PDH was significantly increased after Hb stimulation. (C,D) Quantitative analysis of Western blot and qPCR results showed that variation of PDKs protein at each time point (0, 1, 3, 6, 12, and 24 h) and mRNA at each time point (0, 1, 4, 12, and 24 h) levels after Hb stimulation. (E) Representative immunofluorescence staining for PDK4 and NeuN (a neuronal marker) in cultured primary neurons after Hb stimulation (PDK4, green; NeuN, red; DAPI, blue). Bars represent the means ± SD. * p [less than] 0.05; ** p [less than] 0.01 vs. con (n = 5 in each group). Bar = 50 μm.

Reference

1. Gao, X.; Gao, Y.-Y.; Wu, L.-Y.; Peng, Z.; Liu, X.-Z.; Chen, X.-X.; Gao, S.; Zhang, H.-S.; Lu, Y.; Hang, C.-H. et al. High Expression of PDK4 Could Play a Potentially Protective Role by Attenuating Oxidative Stress after Subarachnoid Hemorrhage. J. Clin. Med.; 2022; 11, 3974. [DOI: https://dx.doi.org/10.3390/jcm11143974] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/35887737]