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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Despite the growing interest in using natural compounds for disease prevention and treatment, Allium ursinum (wild garlic), known for its therapeutic properties, has not been extensively studied for its chemical composition and biological activities. Therefore, this study aims to explore the in vitro antioxidant, antibacterial, and antitumor activities of A. ursinum extracts according to their functional phytochemical profile, while assessing whether ultrasound-assisted extraction (UAE) enhances bioactive properties in comparison to conventional maceration (CM). Both extracts were characterized by spectrophotometric methods and LC-ESI+-MS. The antioxidant activity was assessed via the CUPRAC and hydrogen peroxide scavenging assays, the antimicrobial properties via the disk-diffusion method against five pathogenic strains, and the antitumor activity via the MTT assay on four cancer cell lines. The major constituents of the methanolic extracts from leaves were kaempferol derivatives and alliin. The quercetin derivative rutin was also found. Maceration assisted using UAE yielded 20% more bioactive compounds in comparison to CM alone. Employing UAE in the extraction significantly increased antioxidant and antimicrobial proprieties, in line with its chemical composition. The antitumor cytotoxic activity was low to moderate, regardless of method, as explained by the absence of highly cytotoxic compounds. Wild garlic extracts possessed strong antioxidant and substantial antibacterial activities.

Details

Title
The Antioxidant, Antimicrobial, and Antitumor Proprieties of Flavonol-Rich Extracts from Allium ursinum (Wild Garlic) Leaves: A Comparison of Conventional Maceration and Ultrasound-Assisted Extraction Techniques
Author
Oravetz, Kinga 1   VIAFID ORCID Logo  ; Zorita Diaconeasa 2   VIAFID ORCID Logo  ; Carpa, Rahela 3   VIAFID ORCID Logo  ; Rakosy-Tican, Elena 1 ; Cruceriu, Daniel 4 

 Centre for Systems Biology, Biodiversity and Bioresources, Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babes-Bolyai University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania; [email protected] (K.O.); [email protected] (E.R.-T.); [email protected] (D.C.) 
 Department of Chemistry, Biochemistry and Molecular Biology, University of Agricultural Sciences and Veterinary Medicine, 3-5 Calea Mănăştur, 400372 Cluj-Napoca, Romania; [email protected] 
 Centre for Systems Biology, Biodiversity and Bioresources, Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babes-Bolyai University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania; [email protected] (K.O.); [email protected] (E.R.-T.); [email protected] (D.C.); Institute for Research-Development-Innovation in Applied Natural Sciences, Babes-Bolyai University, 30 Fântânele St., 400294 Cluj-Napoca, Romania 
 Centre for Systems Biology, Biodiversity and Bioresources, Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babes-Bolyai University, 5-7 Clinicilor Street, 400006 Cluj-Napoca, Romania; [email protected] (K.O.); [email protected] (E.R.-T.); [email protected] (D.C.); The Oncology Institute “Prof. Dr. Ion Chiricuta”, Department of Genetics, Genomics and Experimental Pathology, 34-36 Republicii Street, 400015 Cluj-Napoca, Romania 
First page
12799
Publication year
2024
Publication date
2024
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3144192335
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.