Disruptions in Primary Visual Cortex Physiology and Function in a Mouse Model of Timothy Syndrome

Abstract

Timothy syndrome (TS) is a rare genetic disorder caused by mutations in the CACNA1C gene which encodes the L-type calcium channel α-1 CaV1.2 subunit. While it is expressed throughout the body the most serious symptoms are cardiac and neurological. Classical TS1 and TS2 mutations cause prolonged action potentials (APs) in cardiomyocytes and in induced neurons derived from pluripotent stem cells taken from TS patients, but effects of TS mutations on neuronal function in vivo are not fully understood. TS is frequently associated with autistic traits, which in turn have been linked to altered sensory processing. Using the TS2-neo mouse model we analysed effects of the TS2 mutation on the visual system. We observed a widening of APs of pyramidal cells in ex vivo patch-clamp recordings and an increase in the density of parvalbumin positive (PV+) cells in the primary visual cortex. Neurons recorded extracellularly in vivo were less likely to respond to visual stimuli of low spatial frequency, but more likely to respond to visual stimuli of mid-to-high spatial frequency, compared to WT mice. These results point to a basic processing abnormality in the visual cortex of TS2-neo mice.

Competing Interest Statement

The authors have declared no competing interest.