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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Cornea tissue engineering is strictly dependent on the development of biomaterials that fulfill the strict biocompatibility, biomechanical, and optical requirements of this organ. In this work, we generated novel biomaterials from the squid gladius (SG), and their application in cornea tissue engineering was evaluated. Results revealed that the native SG (N-SG) was biocompatible in laboratory animals, although a local inflammatory reaction was driven by the material. Cellularized biomaterials (C-SG) demonstrated that the SG provides an adequate substrate for cell attachment and growth, and corneal epithelial cells cultured on this biomaterial were able to express crystallin alpha, a marker for this type of cells. Biomechanical analyses showed that N-SG biomaterials have higher Young modulus and lower traction deformation than control native corneas (CTR), and C-SG showed a similar Young modulus than CTR. Analysis of the optical properties of these samples revealed that the diffuse transmittance of N-SG and C-SG were higher than CTR, with the diffuse reflectance showing the opposite behavior. These results confirm the putative usefulness of this abundant marine-derived biomaterial that can be obtained as a byproduct of the fishing industry.

Details

Title
Development of Novel Squid Gladius Biomaterials for Cornea Tissue Engineering
Author
Garzón, Ingrid 1 ; Muñoz-Hurtado, Juan 1 ; Pereira-Martínez, Juan 1 ; Ionescu, Ana M 2 ; Juan de la Cruz Cardona 2   VIAFID ORCID Logo  ; Tejada-Casado, María 2 ; María del Mar Pérez 2   VIAFID ORCID Logo  ; Campos, Fernando 1   VIAFID ORCID Logo  ; Chato-Astrain, Jesús 1   VIAFID ORCID Logo  ; Alaminos, Miguel 1   VIAFID ORCID Logo 

 Tissue Engineering Group, Department of Histology, Faculty of Medicine, University of Granada, E18016 Granada, Spain; [email protected] (I.G.); [email protected] (J.M.-H.); [email protected] (J.P.-M.); [email protected] (M.A.); Instituto de Investigación Biosanitaria ibs.GRANADA, E18011 Granada, Spain; [email protected] (A.M.I.); [email protected] (J.d.l.C.C.); [email protected] (M.d.M.P.) 
 Instituto de Investigación Biosanitaria ibs.GRANADA, E18011 Granada, Spain; [email protected] (A.M.I.); [email protected] (J.d.l.C.C.); [email protected] (M.d.M.P.); Laboratory of Biomaterials Optics, Department of Optics, Faculty of Science, University of Granada, E18071 Granada, Spain 
First page
535
Publication year
2024
Publication date
2024
Publisher
MDPI AG
e-ISSN
16603397
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3149697884
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.