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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Elymus sibiricus, valued for its perennial nature, broad adaptability, strong cold tolerance, and high economic value in forage production, plays a crucial role in combating grassland degradation, desertification, and salinization. Using morphological and cytogenetic methods, this study evaluated the cold tolerance, post-harvest regeneration capacity, and perennial characteristics of the E. sibiricus accession 20HSC-Z9 in the Harbin region of China from 2020 to 2023. This accession exhibited a germination rate of over 90% and a 100% green-up rate, with purple coleoptiles indicating its strong cold tolerance. Over the three growing seasons, 20HSC-Z9 maintained stable green-up and regeneration rates, confirming its perennial nature. Morphologically, 20HSC-Z9 had an average tiller count ranging from 56 to 74, similar to that of the control accession 20HSC-ES, and its plant height was significantly lower than that of 20HSC-IWG. Furthermore, 20HSC-Z9 produced over 100 grains per spike, with a seed setting rate exceeding 90%, and a thousand-grain weight comparable to that of 20HSC-IWG. The grain protein content of 20HSC-Z9 reached a maximum of 21.19%, greater than that of the control accessions (15.6% and 18.5%). Chromosome composition analysis, using sequential multicolor genomic in situ hybridization and multicolor fluorescence in situ hybridization, confirmed the StStHH genomic constitution of 20HSC-Z9 and revealed translocations between the St and H subgenome chromosomes. These results suggest that 20HSC-Z9 has significant potential as a new perennial forage grass germplasm for cold regions, suitable for further domestication and breeding efforts.

Details

Title
Characterization of a Natural Accession of Elymus sibiricus with In Situ Hybridization and Agronomic Evaluation
Author
Liu, Yizhuo 1 ; Ding, Jiarui 1 ; Wu, Chunfei 1 ; Song, Weiwei 1 ; Zhao, Xinyu 1 ; Zhao, Haibin 2 ; Qu, Yunfeng 3 ; Jin, Hui 2 ; Zhang, Rui 2 ; Li, Mingyao 1 ; Yan, Xinyu 1 ; Zhu, Liangyu 1 ; Bao, Yaqi 1 ; Liu, Dianhao 1 ; Li, Xinling 1 ; Cui, Lei 4 ; Li, Hongjie 3 ; Zhang, Yanming 1   VIAFID ORCID Logo 

 Key Laboratory of Molecular Cytogenetics and Genetic Breeding of Heilongjiang Province, College of Life Science and Technology, Harbin Normal University, Harbin 150025, China; [email protected] (Y.L.); [email protected] (J.D.); [email protected] (C.W.); [email protected] (W.S.); [email protected] (X.Z.); [email protected] (M.L.); [email protected] (X.Y.); [email protected] (L.Z.); [email protected] (Y.B.); [email protected] (D.L.); [email protected] (X.L.) 
 Institute of Forage and Grassland Sciences, Heilongjiang Academy of Agricultural Sciences, Harbin 150086, China; [email protected] (H.Z.); [email protected] (H.J.); [email protected] (R.Z.) 
 Institute of Biotechnology, Xianghu Laboratory, Hangzhou 311200, China; [email protected] 
 College of Agriculture, Shanxi Agricultural University, Taiyuan 030031, China; [email protected] 
First page
75
Publication year
2025
Publication date
2025
Publisher
MDPI AG
e-ISSN
22237747
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3153692201
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.