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© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Olfactory ensheathing cell (OEC) transplantation demonstrates promising therapeutic results in neurological disorders, such as spinal cord injury. The emerging cell-free secretome therapy compensates for the limitations of cell transplantation, such as low cell survival rates. However, the therapeutic benefits of the human OEC secretome remain unclear. We harvested the secretome from human mucosal OECs and characterized its protein content, identifying 709 proteins in the human OEC secretome from three donors in two passages. Thirty-nine proteins, including neurological-related proteins, such as profilin-1, and antioxidants, such as peroxiredoxin-1 and glutathione S-transferase, were shared between the six samples. The secretome consistently demonstrated potential effects such as antioxidant activity, neuronal differentiation, and quiescence exit of neural stem cells (NSCs). The total secretome produced by OECs protects NSCs from H2O2-induced reactive oxygen species accumulation. During induction of neuronal differentiation, secretomes promoted neurite outgrowth, axon elongation, and expression of neuronal markers. The secretome ameliorated bone morphogenetic protein 4- and fibroblast growth factor 2-induced quiescence of NSCs. The human OEC secretome triggers NSCs to exit prime quiescence, which is related to increased phosphoribosomal protein S6 expression and RNA synthesis. The human OEC secretome has beneficial effects on NSCs and may be applied in neurological disease studies.

Details

Title
Secretome of the Olfactory Ensheathing Cells Influences the Behavior of Neural Stem Cells
Author
Yu-Huan Hsueh 1 ; Kuan-Po Chen 1   VIAFID ORCID Logo  ; Buddhakosai, Waradee 2 ; Phung-Ngan Le 2 ; Ying-Wu, Hsiung 2 ; Yung-Yi Tu 3 ; Wen-Liang, Chen 4 ; Lu, Huai-En 5   VIAFID ORCID Logo  ; Yuan-Kun Tu 2 

 Department of Orthopedic Surgery, E-Da Hospital, I-Shou University, Kaohsiung City 824, Taiwan; College of Engineering Bioscience, National Yang Ming Chiao Tung University, Hsinchu City 300, Taiwan 
 Department of Orthopedic Surgery, E-Da Hospital, I-Shou University, Kaohsiung City 824, Taiwan 
 School of Medicine, National Taiwan University, Taipei City 106, Taiwan 
 Department of Biological Science and Technology, National Yang Ming Chiao Tung University, Hsinchu City 300, Taiwan 
 Bioresource Collection and Research Center, Food Industry Research and Development Institute, Hsinchu City 300, Taiwan; Institute of Biochemistry and Molecular Biology, National Yang Ming Chiao Tung University, Hsinchu City 300, Taiwan; Center for Regenerative Medicine and Cellular Therapy, National Yang Ming Chiao Tung University, Hsinchu City 300, Taiwan 
First page
281
Publication year
2025
Publication date
2025
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3153752845
Copyright
© 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.