Abstract

Porcine parvoviruses (PPVs) are diverse and persistently evolving viruses found in domestic pigs and wild boars. Porcine parvovirus 1 (PPV1) causes reproductive problems in adult animals, although the veterinary relevance of PPV2, PPV3, and PPV4 has not been clarified. The detection and sequence analysis of PPVs circulating in wild boar populations in Serbia was performed to determine their phylogenetic relationships and prevalence in 122 organ samples collected during 2018. The DNA of PPV1, PPV2, and PPV3 was detected in 56.6% of the examined samples, whilst PPV4 was not identified. Overall, PPV3 was the most prevalent in 69.6% of the positive samples, followed by PPV1 in 63.8%, and PPV2 in 21.7% samples. Single infections were more common, although concurrent infections were confirmed in 34.8% samples for two, and 10.1% samples for three viruses. Sequence analysis of wild boar PPV1 showed no significant nucleotide differences from domestic pig PPV1 strains detected in Europe and the USA, however separate clustering from strains from China and the NADL-2 strain was demonstrated. Examination of the selected PPV2 sequences might suggest a certain geographical distribution of genetically diverse PPV2 strains considering high similarities to the strains from neighboring countries, and variability in comparison with other reported PPV2 sequences from different parts of the world. Wild boar PPV3 sequences clustered separately from most of the strains detected in wild boars, as well as the original porcine hokovirus strain. It is further noted that genetically different PPV3 strains circulate amongst Serbian domestic pigs and wild boars.

Details

Title
Genetic Analysis and Distribution of Porcine Parvoviruses Detected in the Organs of Wild Boars in Serbia
Author
Nišavić Jakov 1 ; Milić Nenad 1 ; Radalj Andrea 1 ; Krnjaić Dejan 1 ; Milićević Dragan 2 ; Knežević Aleksandra 3 ; Radojičić Marina 1 ; Obrenović Sonja 4 ; Ćosić Milivoje 5 ; Tešović Bojana 6 ; Benković Damir 7 ; Živulj Aleksandar 8 

 Department of Microbiology, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia 
 Institute of Meat Hygiene and Technology, Belgrade, Serbia 
 Virology Department, Institute of Microbiology and Immunology, Faculty of Medicine, University of Belgrade, Belgrade, Serbia 
 Department of Infectious Animals Diseases and Diseases of Bees, Faculty of Veterinary Medicine, University of Belgrade, Belgrade, Serbia 
 Department of Animal Husbandary, Faculty of Agriculture, University of Bijeljina, Bosnia and Herzegovina 
 Veterinary Specialized Institute „Kraljevo“, Kraljevo, Serbia 
 Veterinary Specialized Institute „Sombor“, Sombor, Serbia 
 Veterinary Specialized Institute “Pančevo”, Pančevo, Serbia 
Pages
32-46
Publication year
2021
Publication date
2021
Publisher
De Gruyter Poland
ISSN
05678315
e-ISSN
18207448
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3155349497
Copyright
© 2021. This work is published under http://creativecommons.org/licenses/by/4.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.