Abstract

Purpose: The implantable bladder cancer (BC) models allow the researchers to perform rapid and useful experiments for BC. We investigated the implantation success of BC cells obtained from Wistar rats (grown in vitro), into bladders of syngeneic Wistar rats, which are commonly used in the laboratories.

Methods: The Nara Bladder Tumor No.2 (NBT-II) BC cells induced with 4-hydroxybutylnitrosamine were grown with passages in Kocaeli University Center for Stem-Cell and Gene-Therapies. After urothelial denudation, 2x106 NBT-II cells were then implanted into bladders of 24 female Wistar rats (aged 7-8 weeks). The rats were randomly divided into four experimental groups; three instillation groups (8 per group) and one sham-operated control group consisting of 6 rats. First, second and third instillation groups were sacrificed at days 7, 14, and 21, respectively, and, bladders were histopathologically evaluated for BC according to WHO / International Society of Urological Pathology.

Results: All tumors were pT1 (including 1 rat that prematurely died at 5th day), except one rat that died prematurely at 8th day had pT2 tumor. Implantation rates were 28.58% (2/7) in the first group, and 42.85% (3/7) in the second, for a cumulative rate of 35.71% (5/14) in these two-groups (until 14th day). Interestingly, there was no tumor in the third group, but there was an inflammatory granulation tissue.

Conclusion: Seeding NBT-II cells into bladders of Wistar rats was described, successfully tested and demonstrated in this study. This implantable BC model of Wistar rats may be improved to increase the success rate of BC cell implantation in new studies with higher number of animals.

Details

Title
Implantable orthotopic bladder cancer model in Wistar rats: A pilot and feasibility study
Author
Teke, Kerem 1 ; Yilmaz, Hasan 1 ; Kosem, Mehmet Esad 1 ; Yuksekkaya, Mustafa 1 ; Yusufoglu, Sema 2 ; Bayrak, Busra Yaprak 3 ; Yusufhan Yazir 2 ; Yildiz, Demir Kursat 3 ; Dillioglugil, Ozdal 1 

 Department of Urology, Kocaeli University School of Medicine, Kocaeli, Turkey 
 Center for Stem Cell and Gene Therapies, Kocaeli University School of Medicine, Kocaeli, Turkey 
 Department of Pathology, Kocaeli University School of Medicine, Kocaeli, Turkey 
Pages
35-42
Publication year
2019
Publication date
2019
Publisher
De Gruyter Poland
ISSN
18416624
e-ISSN
22845623
Source type
Scholarly Journal
Language of publication
Multiple languages; English
ProQuest document ID
3157777750
Copyright
© 2019. This work is published under http://creativecommons.org/licenses/by-nc-nd/3.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.