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Abstract
The capacity of the F3K Digitalis purpurea cell line to biotransform hydroquinone into β-arbutin was tested. The experimental protocol involved the sequential addition of hydroquinone to the culture medium, and testing different precursor (4-, 5-, and 6 mM) and sucrose concentrations (3 % and 6 %) under different lighting conditions (16-hour photoperiod and darkness). The biotransformation process was positively influenced by the photoperiod regime and increased sucrose concentration. The highest arbutin production (1 g/L) has been achieved in cell suspensions fed with 6% sucrose and 5 mM hydroquinone, under 16 hours daily light exposure.
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Details
1 Department of Drug Industry and Pharmaceutical Biotechnology, Faculty of Pharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, Romania
2 Department of Pharmaceutical Botany, Iuliu Haţieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, Romania
3 Institute of Biological Research Cluj subsidiary, National Institute of Research and Development for Biological Sciences, 48 Republicii Street, 400015 Cluj‑Napoca, Romania
4 Department of Pharmaceutical Technology and Biopharmacy, Iuliu Haţieganu University of Medicine and Pharmacy, 8 Victor Babeș Street, 400012 Cluj-Napoca, Romania