Abstract

Juglans is an economically important genus distributed all over the world. Due to its high value, there is a large interest in genetic markers for population genetic analyses and the certification of reproductive material. Using chloroplast genomes of different Juglans species, we developed a highly polymorphic and reliable chloroplast SSR marker set of eleven cpSSRs. It was successfully tested in five different Juglans species. Genetic differentiation among species and provenances within species was assessed. We were able to define a total of nine different chloroplast haplotypes using the novel cpSSR markers with up to four different haplotypes within a single Juglans species. Due to the cost and labor effective character of SSR markers, when compared to other available methods, such as whole chloroplast genome sequencing or SNP analyses, this novel cpSSR marker set will contribute to future population genetic studies in the Juglans genus.

Details

Title
A Novel Set of Chloroplast SSR Markers for the Genus Juglans Reveals Within Species Differentiation
Author
Götz, Jeremias 1 ; Caré, Oliver 2 ; Beck, Winston 3 ; Gailing, Oliver 4 ; Hosius, Bernhard 1 ; Leinemann, Ludger 2 

 ISOGEN GmbH & Co. KG; Büsgenweg 2; 37077 Göttingen, Germany 
 ISOGEN GmbH & Co. KG; Büsgenweg 2; 37077 Göttingen, Germany; Department of Forest Genetics and Forest Tree Breeding; Georg-August University of Göttingen; Büsgenweg 2; 37077 Göttingen, Germany 
 Division Urban Plant Ecophysiology, Humboldt-Universität zu Berlin, Lentzeallee 55-57, 14195 Berlin, Germany 
 Department of Forest Genetics and Forest Tree Breeding; Georg-August University of Göttingen; Büsgenweg 2; 37077 Göttingen, Germany 
Pages
120-125
Publication year
2024
Publication date
2024
Publisher
De Gruyter Poland
ISSN
00375349
e-ISSN
25098934
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3159620107
Copyright
© 2024. This work is published under http://creativecommons.org/licenses/by-nc-nd/3.0 (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.