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© 2025. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

ABSTRACT

Background

Acute lung injury (ALI), one of the most severe respiratory system diseases, is prevalent worldwide. Annexin A1 (AnxA1) is an important member of the annexin superfamily, known for its wide range of physiological functions. However, its potential protective effect against lipopolysaccharide (LPS)‐induced ALI remains unclear.

Materials and Methods

Mice were divided into four groups: Sham, LPS + vehicle, LPS + 0.1 μg AnxA1, and LPS + 0.5 μg AnxA1. Lung injury was assessed through histopathology, pulmonary wet‐to‐dry (W/D) ratio, cell counting of bronchoalveolar lavage fluid (BALF), oxidative stress analysis, and noninvasive pulmonary function testing. Gene and protein expression levels were measured using RT‐PCR, ELISA, and western blot analysis.

Results

AnxA1 alleviated LPS‐induced ALI by protecting lung tissue from damage, reducing the lung wet/dry (W/D) weight ratio, and improving LPS‐induced impaired lung function. Interestingly, administration of AnxA1 was found to repress the infiltration of inflammatory cells by decreasing the total cell count, neutrophils, and protein concentrations in bronchoalveolar lavage fluid (BALF). AnxA1 mitigated the inflammatory response in the pulmonary tissue by lowering the levels of IL‐1β, IL‐6, and TNF‐α in BALF of ALI mice. Additionally, AnxA1 attenuated oxidative stress in lung tissues of ALI mice by restoring the activity of catalase (CAT), SOD, and glutathione (GSH) but reducing the levels of malondialdehyde (MDA). We also found that AnxA1 suppressed activation of the NLRP3 signaling pathway. Mechanistically, AnxA1 activated the Nrf2/HO‐1 signaling pathway while preventing the activation of NF‐κB.

Conclusion

Collectively, these findings suggest that AnxA1 alleviates LPS‐induced ALI and might be a promising novel therapeutic agent against LPS‐induced ALI.

Details

Title
The Protective Effects of Annexin A1 in Acute Lung Injury Mediated by Nrf2
Author
Huang, Hui 1 ; Shi, Yuqin 2 ; Zhou, Yuequan 2   VIAFID ORCID Logo 

 Department of Stomatology, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China 
 Department of Respiratory and Critical Care Medicine, Liyuan Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, China 
Section
ORIGINAL ARTICLE
Publication year
2025
Publication date
Jan 1, 2025
Publisher
John Wiley & Sons, Inc.
e-ISSN
20504527
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3160661054
Copyright
© 2025. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.