Abstract

Background

Shigella is a major cause of diarrhea in young children worldwide. Multiple vaccines targeting Shigella are in development, and phase 3 clinical trials are imminent to determine efficacy against shigellosis.

Methods

The Enterics for Global Health (EFGH) Shigella surveillance study is designed to determine the incidence of medically attended shigellosis in 6- to 35-month-old children in 7 resource-limited settings. Here, we describe the microbiological methods used to isolate and identify Shigella. We developed a standardized laboratory protocol for isolation and identification of Shigella by culture. This protocol was implemented across all 7 sites, ensuring consistency and comparability of results. Secondary objectives of the study are to determine the antibiotic resistance profiles of Shigella, compare isolation of Shigella from rectal swabs versus whole stool, and compare isolation of Shigella following transport of rectal swabs in Cary-Blair versus a modified buffered glycerol saline transport medium.

Conclusions

Data generated from EFGH using culture methods described herein can potentially be used for microbiological endpoints in future phase 3 clinical trials to evaluate vaccines against shigellosis and for other clinical and public health studies focused on these organisms.

Details

Title
Microbiological Methods Used in the Enterics for Global Health Shigella Surveillance Study
Author
Bri’Anna Horne 1 ; Badji, Henry 2 ; Md Taufiqur Rahman Bhuiyan 3 ; Lucero Romaina Cachique 4 ; Cornick, Jennifer 5 ; Hotwani, Aneeta 6 ; Juma, Jane 7 ; Ochieng, John Benjamin 8 ; Abdou, Mahamadou 7 ; Evans Apondi 8 ; Atlas, Hannah E 9 ; Awuor, Alex O 8 ; Baker, Kate S 5 ; Ceesay, Bubacarr E 2 ; Charles, Mary 10 ; Cunliffe, Nigel A 5 ; Feutz, Erika 9 ; Galagan, Sean R 9 ; Guindo, Ibrehima 7 ; M Jahangir Hossain 2 ; Iqbal, Junaid 6 ; Jallow, Fatima 2 ; Keita, Noumou Yakhouba 7 ; Khanam, Farhana 3 ; Kotloff, Karen L 1 ; Maiden, Victor 10 ; Katia Manzanares Villanueva 4 ; Mito, Oscar 8 ; Mosharraf, Md Parvej 3 ; Nkeze, Joseph 1 ; Ikumapayi, Usman N 2 ; Maribel Paredes Olortegui 4 ; Pavlinac, Patricia B 9 ; Tackeshy Pinedo Vasquez 4 ; Qadri, Firdausi 3 ; Farah Naz Qamar 6 ; Qureshi, Sonia 6 ; Rahman, Nazia 3 ; Sangare, Aminata 7 ; Sen, Sunil 1 ; Yori, Pablo Peñataro 11 ; Mohammad Tahir Yousafzai 6 ; Ahmed, Dilruba 3 ; Jere, Khuzwayo C 5 ; Kosek, Margaret N 11 ; Omore, Richard 8 ; Permala-Booth, Jasnehta 1 ; Ousman Secka 2 ; Tennant, Sharon M 1 

 Center for Vaccine Development and Global Health, University of Maryland School of Medicine , Baltimore, Maryland , USA 
 Medical Research Council Unit The Gambia, London School of Hygiene and Tropical Medicine , Fajara , The Gambia 
 International Centre for Diarrhoeal Disease Research, Bangladesh , Dhaka , Bangladesh 
 Asociación Benéfica PRISMA , Iquitos, Loreto , Peru 
 Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool , Liverpool , United Kingdom 
 Department of Pediatrics and Child Health, The Aga Khan University , Karachi , Pakistan 
 Centre pour le Développement des Vaccins du Mali , Bamako , Mali 
 Center for Global Health Research, Kenya Medical Research Institute , Kisumu , Kenya 
 Department of Global Health, University of Washington , Seattle, Washington , USA 
10  Malawi Liverpool Wellcome Research Programme , Blantyre , Malawi 
11  Division of Infectious Diseases and International Health, University of Virginia , Charlottesville, Virginia , USA 
Pages
S25-S33
Publication year
2024
Publication date
Mar 2024
Publisher
Oxford University Press
e-ISSN
23288957
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3168667816
Copyright
© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.