Abstract

Background

Quantitative polymerase chain reaction (qPCR) targeting ipaH has been proven to be highly efficient in detecting Shigella in clinical samples compared to culture-based methods, which underestimate Shigella burden by 2- to 3-fold. qPCR assays have also been developed for Shigella speciation and serotyping, which is critical for both vaccine development and evaluation.

Methods

The Enterics for Global Health (EFGH) Shigella surveillance study will utilize a customized real-time PCR–based TaqMan Array Card (TAC) interrogating 82 targets, for the detection and differentiation of Shigella spp, Shigella sonnei, Shigella flexneri serotypes, other diarrhea-associated enteropathogens, and antimicrobial resistance (AMR) genes. Total nucleic acid will be extracted from rectal swabs or stool samples, and assayed on TAC. Quantitative analysis will be performed to determine the likely attribution of Shigella and other particular etiologies of diarrhea using the quantification cycle cutoffs derived from previous studies. The qPCR results will be compared to conventional culture, serotyping, and phenotypic susceptibility approaches in EFGH.

Conclusions

TAC enables simultaneous detection of diarrheal etiologies, the principal pathogen subtypes, and AMR genes. The high sensitivity of the assay enables more accurate estimation of Shigella-attributed disease burden, which is critical to informing policy and in the design of future clinical trials.

Details

Title
Shigella Detection and Molecular Serotyping With a Customized TaqMan Array Card in the Enterics for Global Health (EFGH): Shigella Surveillance Study
Author
Liu, Jie 1 ; Garcia Bardales, Paul F 2 ; Islam, Kamrul 3 ; Sheikh Jarju 4 ; Juma, Jane 5 ; Mhango, Chimwemwe 6 ; Queen Naumanga 7 ; Qureshi, Sonia 8 ; Sonye, Catherine 9 ; Ahmed, Naveed 8 ; Aziz, Fatima 8 ; Md Taufiqur Rahman Bhuiyan 3 ; Charles, Mary 6 ; Cunliffe, Nigel A 10 ; Abdou, Mahamadou 5 ; Galagan, Sean R 11 ; Gitteh, Ensa 4 ; Guindo, Ibrehima 5 ; M Jahangir Hossain 4 ; Abdoulie M J Jabang 4 ; Jere, Khuzwayo C 6 ; Kawonga, Flywell 6 ; Keita, Mariama 4 ; Keita, Noumou Yakhouba 5 ; Kotloff, Karen L 12 ; Wagner V Shapiama Lopez 2 ; Munga, Stephen 9 ; Maribel Paredes Olortegui 2 ; Omore, Richard 9 ; Pavlinac, Patricia B 11 ; Qadri, Firdausi 3 ; Farah Naz Qamar 8 ; S M Azadul Alam Raz 3 ; Riziki, Laura 9 ; Schiaffino, Francesca 7 ; Stroup, Suzanne 7 ; Traore, Sarata Nassoun 5 ; Tackeshy Pinedo Vasquez 2 ; Mohammad Tahir Yousafzai 8 ; Martin, Antonio 4 ; Cornick, Jennifer E 6 ; Kabir, Furqan 8 ; Khanam, Farhana 3 ; Kosek, Margaret N 7 ; Ochieng, John Benjamin 9 ; Platts-Mills, James A 7 ; Tennant, Sharon M 12 ; Houpt, Eric R 7 

 School of Public Health, Qingdao University , Qingdao , China 
 Asociación Benéfica PRISMA , Iquitos, Loreto , Peru 
 Infectious Diseases Division, International Centre for Diarrhoeal Disease Research, Bangladesh (icddr,b), Dhaka , Bangladesh 
 Medical Research Council Unit The Gambia, London School of Hygiene and Tropical Medicine , Fajara , The Gambia 
 Centre pour le Développement des Vaccins du Mali (CVD-Mali), Bamako , Mali 
 Malawi Liverpool Wellcome Research Programme , Blantyre , Malawi 
 Division of Infectious Diseases and International Health, University of Virginia , Charlottesville, Virginia , USA 
 Department of Pediatrics and Child Health, The Aga Khan University , Karachi , Pakistan 
 Center for Global Health Research, Kenya Medical Research Institute , Kisumu , Kenya 
10  Institute of Infection, Veterinary and Ecological Sciences, Department of Clinical Infection, Microbiology and Immunology, University of Liverpool , Liverpool , United Kingdom 
11  Department of Global Health, University of Washington , Seattle, Washington , USA 
12  Center for Vaccine Development and Global Health, University of Maryland School of Medicine , Baltimore, Maryland , USA 
Pages
S34-S40
Publication year
2024
Publication date
Mar 2024
Publisher
Oxford University Press
e-ISSN
23288957
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3168668095
Copyright
© The Author(s) 2024. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.