Correction: Zhao et al. Fasciola gigantica Recombinant Abelson Tyrosine Protein Kinase (rFgAbl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells. Animals 2025, 15, 179

Error in Figure 4

In the original publication [1], there was a mistake in Figure 4 as published. Some of the unpublished materials (fluorescent image of FgBMP-1) were included in the figure which should have been removed. There was a mistake in the Figure 4 legend as published. Some of the unpublished materials (fluorescent image of FgBMP-1) were included in the figure which should have been removed. The corrected Figure 4 appears below.

Author Name and Order Change

In the published publication, there was an error regarding the name of Yu Zou. The order of the authors has been updated as follows: Min Zhao, Yu Zou, Wanting Chen, Dongqi Wu, Chengjun Xian, Haoqing Yang, Jiacheng Tan, Wenda Di, Wende Wu and Dongying Wang.

Text Correction

There was an error in the original publication. Some of the unpublished materials were included in the text which should have been removed.

1. A correction has been made to the Materials and Methods Section, 2.9. Immunofluorescence Detection of Recombinant Fasciola gigantica (Fg) Abelson Tyrosine Protein Kinase (Abl) Protein Binding to Buffaloe Peripheral Blood Mononuclear Cells (PBMCs), first paragraph:

“Immunofluorescence assay (IFA) was performed according to previous studies [21]. Briefly, we added 1 mL of PBMC, rFgAbl protein and PBS for the blank control group to the 12-well plate. We incubated the cells for 6 h at 37 °C in a 5% CO₂ incubator. We washed the cells five times with PBS and fixed the cells with paraformaldehyde for 30 min at 37 °C. We washed the cells five times with PBS and blocked with 5% BSA in PBS at 37 °C for 1 h. We incubated the rabbit anti-rFgAbl polyclonal antibody with the rFgAbl-treated and PBS group PBMCs for 12 h at 4 °C.”

2. Results Section, 3.4. Binding Affinity of rFgAbl Protein to Buffaloe Peripheral Blood Mononuclear Cells (PBMCs), first paragraph:

“Following incubation with rabbit anti-rFgAbl and Cy3-labeled goat anti-rabbit IgG (which emits red fluorescence), as shown in Figure 4, red complexes were detected on the surface of the PBMCs, while DAPI-stained nuclei exhibited blue fluorescence. No red fluorescence was observed in the untreated control group.”

References

With this correction, one of the references has been removed (reference 21—unpublished materials) and replaced with another one: Ehsan, M.; Hu, R.S.; Hou, J.L.; Elsheikha, H.M.; Li, X.D.; Liang, P.H.; Zhu, X.Q. Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes. Parasit. Vectors 2021, 14, 276.

The authors state that the scientific conclusions are unaffected. This correction was approved by the Academic Editor. The original publication has also been updated.

Footnotes

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Figure

Figure 4. The recombinant F. giganticaAbelson tyrosine protein kinase (rFgAbl) binds to the surface of buffalo peripheral blood mononuclear cells (PBMCs). PBMCs treated and untreated with rFgAbl were incubated with rabbit anti-rFgAbl antibody and stained with Cy3-conjugated goat anti-rabbit IgG. PBMCs surface staining was observed in cells treated with rFgAbl, whereas no staining was detected in untreated cells. Scale bar: 50 μm.

Reference

1. Zhao, M.; Zou, Y.; Chen, W.; Wu, D.; Xian, C.; Yang, H.; Tan, J.; Wu, W.; Di, W.; Wang, D. Fasciola gigantica Recombinant Abelson Tyrosine Protein Kinase (rFgAbl) Regulates Various Functions of Buffalo Peripheral Blood Mononuclear Cells. Animals; 2025; 15, 179. [DOI: https://dx.doi.org/10.3390/ani15020179] [PubMed: https://www.ncbi.nlm.nih.gov/pubmed/39858178]