Abstract

Glucagon-like peptide-1 (GLP-1) and peptide YY (PYY), produced by intestinal enteroendocrine L cells, are important gut hormones that coordinate gastrointestinal physiology, metabolism, and appetite. We aimed to investigate the role of olfactory receptor (OR) OR51E1 in GLP-1 and PYY secretion. We analyzed the expression of olfactory marker protein (OMP), an indicator of OR-mediated events in nonolfactory systems, in human intestinal L cells. Furthermore, we analyzed OMP and OR51E1 expression in the L cell line NCI-H716. To investigate whether odorant-activated OR signaling stimulates GLP-1 and PYY secretion, we used nonanoic acid, a known OR51E1 ligand. Treatment with 100 μM nonanoic acid increased GLP-1 secretion by 2.32 ± 0.41-fold and PYY secretion by 1.44 ± 0.10-fold; however, this effect was attenuated on small interfering RNA-mediated OR51E1 knockdown. Oral administration of nonanoic acid to rats resulted in a 2.89 ± 0.53-fold increase in GLP-1 levels and reductions in blood glucose levels compared with the control group. Nonanoic acid stimulates GLP-1 and PYY secretion via OR51E1 signaling in L cells, thereby indicating a potential role of OR-mediated events in GLP-1 and PYY secretion; this could be translated into a therapeutic approach in treating diabetes.

Details

Title
Olfactory Receptor OR51E1 Mediates GLP-1 Secretion in Human and Rodent Enteroendocrine L Cells
Author
Han, Ye Eon 1 ; Chan Woo Kang 1 ; Oh, Joo Heon 1 ; Park, Se Hee 2 ; Ku, Cheol Ryong 3 ; Cho, Yoon Hee 3 ; Lee, Mi Kyung 4 ; Eun Jig Lee 1 

 Brain Korea 21 PLUS Project for Medical Science, Yonsei University, Seoul, Republic of Korea; Endocrinology, Institute of Endocrine Research, Yonsei University College of Medicine, Seoul, Republic of Korea 
 Department of Endocrinology, National Health Insurance Service Ilsan Hospital, Gyeonggi-do, Republic of Korea 
 Endocrinology, Institute of Endocrine Research, Yonsei University College of Medicine, Seoul, Republic of Korea 
 Department of Pathology, National Health Insurance Service Ilsan Hospital, Gyeonggi-do, Republic of Korea 
Pages
1251-1258
Publication year
2018
Publication date
Nov 2018
Publisher
Oxford University Press
e-ISSN
24721972
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3170627193
Copyright
Copyright © 2018 Endocrine Society. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.