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Abstract
Quantitative reverse-transcription polymerase chain reaction (qRT-PCR) is the most sensitive quantitative diagnostic assay for detection of Ebola virus in multiple body fluids. Despite the strengths of this assay, we present 2 cases of Ebola virus disease (EVD) and highlight the potential for false-negative results during the early and late stages of EVD. The first case emphasizes the low negative-predictive value of qRT-PCR during incubation and the early febrile stage of EVD, and the second case emphasizes the potential for false-negative results during recovery and late neurologic complications of EVD. Careful interpretation of test results are needed to guide difficult admission and discharge decisions in suspected or confirmed EVD.
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Details
1 Médecins Sans Frontières, Operational Center of Brussels, Belgium; Department of International Health, School of Public Health, Johns Hopkins University, Baltimore, Maryland
2 Médecins Sans Frontières, Operational Center of Brussels, Belgium; Department of Infectious Diseases and Tropical Medicine, J.W. Goethe-University Hospital, Frankfurt, Germany
3 Laboratory of Virology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rocky Mountain Laboratories, Hamilton, Montana
4 Médecins Sans Frontières, Operational Center of Brussels, Belgium
5 Clinton Health Initiative, Monrovia, Liberia
6 Médecins Sans Frontières, Operational Center of Brussels, Belgium; Department of Critical Care Medicine, Clinical Center, National Institutes of Health, Bethesda, Maryland