Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) subgenomic RNA (sgRNA) may indicate actively replicating virus, but sgRNA abundance has not been systematically compared between SARS-CoV-2 variants. sgRNA was quantified in 169 clinical samples by real-time reverse-transcription polymerase chain reaction, demonstrating similar relative abundance among known variants. Thus, sgRNA detection can identify individuals with active viral replication regardless of variant.

Details

Title
Subgenomic RNA Abundance Relative to Total Viral RNA Among Severe Acute Respiratory Syndrome Coronavirus 2 Variants
Author
Maxwell, Su 1 ; Ping, Sara 1 ; Nguyen, Phuong-Vi 1 ; Rojas, Alejandra 2 ; Hussaini, Laila 3 ; Ludy Registre Carmola 4 ; Azmain Taz 4 ; Sullivan, Julie 5 ; Martin, Greg S 5 ; Piantadosi, Anne 1   VIAFID ORCID Logo  ; Martinez, Magaly 6 ; Lam, Wilbur A 3 ; Anderson, Evan J 1   VIAFID ORCID Logo  ; Waggoner, Jesse J 1   VIAFID ORCID Logo 

1  Division of Infectious Diseases, Department of Medicine, Emory University , Atlanta, Georgia , USA 
2  Departamento de Producción, Universidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud , Asunción , Paraguay 
3  Department of Pediatrics and Children’s Healthcare of Atlanta, Emory University , Atlanta, Georgia , USA 
4  Department of Pathology, Emory University , Atlanta, Georgia , USA 
5  Atlanta Center for Microsystems–Engineered Point-of-Care Technologies , Atlanta, Georgia , USA 
6  Departamento de Biología Molecular y Biotecnología, Universidad Nacional de Asunción, Instituto de Investigaciones en Ciencias de la Salud , Asunción , Paraguay 
Publication year
2022
Publication date
Nov 2022
Publisher
Oxford University Press
e-ISSN
23288957
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1093/ofid/ofac619
ProQuest document ID
3171169862
Copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of Infectious Diseases Society of America. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.