Abstract

Yeast cells have controllable biosorption on metallic ions during metabolism. However, few studies were dedicated to using yeast-regulated biomimetic mineralization process to control the strontium-doped positions in calcium phosphate microcapsules. In this study, the yeast cells were allowed to pre-adsorb strontium ions metabolically and then served as sacrificing template for the precipitation and calcination of mineral shell. The pre-adsorption enabled the microorganism to enrich of strontium ions into the inner part of the microcapsules, which ensured a slow-release profile of the trace element from the microcapsule. The co-culture with human marrow stromal cells showed that gene expressions of alkaline phosphatase and Collagen-I were promoted. The promotion of osteogenic differentiation was further confirmed in the 3D culture of cell-material complexes. The strategy using living microorganism as ‘smart doping apparatus’ to control incorporation of trace element into calcium phosphate paved a pathway to new functional materials for hard tissue regeneration.

Details

Title
Controlling the strontium-doping in calcium phosphate microcapsules through yeast-regulated biomimetic mineralization
Author
Huang, Miaojun 1 ; Li, Tianjie 2 ; Pan, Ting 2 ; Zhao, Naru 3 ; Yao, Yongchang 4 ; Zhai, Zhichen 4 ; Zhou, Jiaan 5 ; Du, Chang 2 ; Wang, Yingjun 1 

 School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China;; National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China 
 School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China;; Ministry of Education Key Laboratory of Biomedical Materials Science and Engineering, Guangzhou 510006, China 
 School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China;; School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China; 
 School of Materials Science and Engineering, South China University of Technology, Guangzhou 510641, China; 
 National Engineering Research Center for Tissue Restoration and Reconstruction, Guangzhou 510006, China 
Pages
269-276
Publication year
2016
Publication date
Oct 2016
Publisher
Oxford University Press
ISSN
20563418
e-ISSN
20563426
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3171503412
Copyright
© The Author(s) 2016. Published by Oxford University Press. This work is published under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.