Dynamic changes of key enzymes activity in a bromate and perchlorate-reducing hydrogen-based membrane biofilm reactor

Abstract

In this study, the microorganisms in different stages of MBfR were cultured, by using kits to detect the dynamic changes of key enzymes activity, and exploring the effects of biofilm microorganisms on key enzymes activity of MBfR. The results indicated that function or role played by microorganism lived on the outer surface of membrane directly caused the removal of NO₃^--N, BrO₃^- and ClO₄^- from water in the MBfR. The key enzymes activity is related to NO₃^--N and degraded substances. When the concentration of NO₃^--N is 5mg/L, the activities of denitrification enzyme were obviously inhibited.