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Abstract
This study assessed the impact of the urease inhibitor Limus on the microbiological properties of soil during barley cultivation. It examined the activity of microbial groups associated with the nitrogen cycle (ammonifiers, denitrifiers), urease enzyme activity, microbial biomass, microbial respiration, and microbial metabolic quotient. It was found that the application of the inhibitor led to a fourfold reduction in gaseous ammonia emissions on the following day of the experiment and a suppression of enzymatic activity by 47% on the third day of the experiment compared to treatment with urea alone. Microbial biomass remained stable, and the application of the inhibitor led to a temporary suppression of basal respiration intensity, which returned to the control level by the third day.
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