Abstract

The current study aims to develop a safe and highly immunogenic COVID-19 vaccine. The novel combination of a DNA vaccine encoding the full-length Spike (S) protein of SARS-CoV-2 and a recombinant S1 protein vaccine induced high level neutralizing antibody and T cell immune responses in both small and large animal models. More significantly, the co-delivery of DNA and protein components at the same time elicited full protection against intratracheal challenge of SARS-CoV-2 viruses in immunized rhesus macaques. As both DNA and protein vaccines have been proven safe in previous human studies, and DNA vaccines are capable of eliciting germinal center B cell development, which is critical for high-affinity memory B cell responses, the DNA and protein co-delivery vaccine approach has great potential to serve as a safe and effective approach to develop COVID-19 vaccines that provide long-term protection.

Details

Title
A novel DNA and protein combination COVID-19 vaccine formulation provides full protection against SARS-CoV-2 in rhesus macaques
Author
Li, Yuzhong 1 ; Bi, Yanwei 1 ; Xiao, Hongjian 1 ; Yao, Yueting 1 ; Liu, Xiaojuan 1 ; Hu, Zhengrong 1 ; Duan, Jinmei 1 ; Yang, Yaoyun 1 ; Li, Zhihua 1 ; Li, Yadong 1 ; Zhang, Heng 1 ; Chen, Ding 1 ; Yang, Jianbo 1 ; Li, Haiwei 1 ; He, Zhanlong 1 ; Liu, Longding 1 ; Hu, Guangnan 2 ; Liu, Shuying 3 ; Che, Yanchun 1 ; Wang, Shixia 2 ; Li, Qihan 1 ; Lu, Shan 2 ; Wei Cun 1 

1  Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College, Kunming, People’s Republic of China 
2  Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA 
3  SYL-Consulting, Thousand Oaks, CA, USA 
Pages
342-355
Publication year
2021
Publication date
Dec 2021
Publisher
Taylor & Francis Ltd.
e-ISSN
22221751
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1080/22221751.2021.1887767
ProQuest document ID
3190429208
Copyright
© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.