Abstract

SARS-CoV-2 mutations appeared recently and can lead to conformational changes in the spike protein and probably induce modifications in antigenicity. We assessed the neutralizing capacity of antibodies to prevent cell infection, using a live virus neutralization test with different strains [19A (initial one), 20B (B.1.1.241 lineage), 20I/501Y.V1 (B.1.1.7 lineage), and 20H/501Y.V2 (B.1.351 lineage)] in serum samples collected from different populations: two-dose vaccinated COVID-19-naive healthcare workers (HCWs; Pfizer-BioNTech BNT161b2), 6-months post mild COVID-19 HCWs, and critical COVID-19 patients. No significant difference was observed between the 20B and 19A isolates for HCWs with mild COVID-19 and critical patients. However, a significant decrease in neutralization ability was found for 20I/501Y.V1 in comparison with 19A isolate for critical patients and HCWs 6-months post infection. Concerning 20H/501Y.V2, all populations had a significant reduction in neutralizing antibody titers in comparison with the 19A isolate. Interestingly, a significant difference in neutralization capacity was observed for vaccinated HCWs between the two variants but not in the convalescent groups.

Details

Title
Live virus neutralization testing in convalescent patients and subjects vaccinated against 19A, 20B, 20I/501Y.V1 and 20H/501Y.V2 isolates of SARS-CoV-2
Author
Saade, Carla 1   VIAFID ORCID Logo  ; Gonzalez, Claudia 1   VIAFID ORCID Logo  ; Bal, Antonin 1   VIAFID ORCID Logo  ; Valette, Martine 1 ; Saker, Kahina 2   VIAFID ORCID Logo  ; Bruno, Lina 1   VIAFID ORCID Logo  ; Josset, Laurence 1   VIAFID ORCID Logo  ; Mary-Anne Trabaud 2   VIAFID ORCID Logo  ; Thiery, Guillaume 3   VIAFID ORCID Logo  ; Botelho-Nevers, Elisabeth 4   VIAFID ORCID Logo  ; Paul, Stéphane 5   VIAFID ORCID Logo  ; Verhoeven, Paul 6   VIAFID ORCID Logo  ; Bourlet, Thomas 6   VIAFID ORCID Logo  ; Pillet, Sylvie 6   VIAFID ORCID Logo  ; Morfin, Florence 1   VIAFID ORCID Logo  ; Trouillet-Assant, Sophie 1   VIAFID ORCID Logo  ; Pozzetto, Bruno 6   VIAFID ORCID Logo  ; on behalf of COVID-SER study group

1  Laboratoire de Virologie, Institut des Agents Infectieux, Laboratoire associé au Centre National de Référence des virus des infections respiratoires, Hospices Civils de Lyon, Lyon, France; Virpath, CIRI, INSERM U1111, CNRS UMR5308, ENS Lyon, Université Claude Bernard Lyon 1, Villeurbanne, France 
2  Laboratoire de Virologie, Institut des Agents Infectieux, Laboratoire associé au Centre National de Référence des virus des infections respiratoires, Hospices Civils de Lyon, Lyon, France 
3  Service de médecine intensive réanimation, Centre Hospitalier Universitaire de Saint-Etienne, Saint-Etienne, France 
4  CIRI, équipe GIMAP, Université de Lyon, Université de Saint-Etienne, INSERM U1111, CNRS UMR5308, ENS de Lyon, UCBL1, Saint-Etienne, France; Service d'Infectiologie, Centre Hospitalier Universitaire de Saint-Etienne, 42055 Saint-Etienne, France 
5  CIRI, équipe GIMAP, Université de Lyon, Université de Saint-Etienne, INSERM U1111, CNRS UMR5308, ENS de Lyon, UCBL1, Saint-Etienne, France; Département d’immunologie, Centre Hospitalier Universitaire de Saint-Etienne, Saint-Etienne, France 
6  CIRI, équipe GIMAP, Université de Lyon, Université de Saint-Etienne, INSERM U1111, CNRS UMR5308, ENS de Lyon, UCBL1, Saint-Etienne, France; Département des agents infectieux et d’hygiène, Centre Hospitalier Universitaire de Saint-Etienne, Saint-Etienne, France 
Pages
1499-1502
Publication year
2021
Publication date
Dec 2021
Publisher
Taylor & Francis Ltd.
e-ISSN
22221751
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1080/22221751.2021.1945423
ProQuest document ID
3190429567
Copyright
© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group, on behalf of Shanghai Shangyixun Cultural Communication Co., Ltd. This work is licensed under the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.