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© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

FK506-binding proteins (FKBPs) belong to the peptidyl-prolyl cis/trans isomerase (PPIase) superfamily and are involved in a wide range of biological processes including protein folding, hormone signaling, plant growth, and stress responses. However, the FKBPs and their biological functions have not been identified in tea plants. In this study, 21 FKBP genes were identified using the conserved FK506-binding domain (PF00254) in the tea-plant genome. Their phylogeny, classification, structure, motifs, interactors, and expression patterns were analyzed. Comprehensive qRT-PCR analysis revealed distinct expression patterns of CsFKBPs in different tissues and in response to low temperature. Through a comprehensive genome-wide analysis, we characterized the low-temperature expression dynamics of the CsFKBP53 gene family and demonstrated that its overexpression significantly enhances cold tolerance in Arabidopsis. Notably, the transcript levels of CsFKBP53 exhibited pronounced variability across distinct tea (Camellia sinensis) cultivars under cold-stress conditions. These findings not only underscore the functional conservation of FKBP-type immunophilins across plant lineages but also highlight the biotechnological potential of CsFKBP53 as a genetic modulator of low-temperature resilience in crops. By integrating comparative genomics and functional validation, our study establishes a foundation for leveraging conserved stress-response mechanisms to engineer climate-resilient plants.

Details

Title
Genome-Wide Analysis of Tea FK506-Binding Proteins (FKBPs) Reveals That CsFKBP53 Enhances Cold-Stress Tolerance in Transgenic Arabidopsis thaliana
Author
Ming-Hui, Xu 1 ; Tang, Jie 1 ; Cai-Ning, Liu 1 ; Wan-Qiao, Zhang 1 ; Li, Qian 1 ; Yang, Fan 2 ; Dan-Dan, Liu 1   VIAFID ORCID Logo 

 School of Agriculture, Yunnan University, Kunming 650091, China; [email protected] (M.-H.X.); [email protected] (J.T.); [email protected] (C.-N.L.); [email protected] (W.-Q.Z.); [email protected] (Q.L.) 
 Yunnan International Joint R&D Center for Intergrated Utilization of Ornamental Grass, College of Landscape and Horticulture, Southwest Forestry University, Kunming 650224, China 
First page
3575
Publication year
2025
Publication date
2025
Publisher
MDPI AG
ISSN
16616596
e-ISSN
14220067
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3194614280
Copyright
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.