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© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Sunflower Verticillium Wilt and Leaf Mottle (SVW), caused by Verticillium dahliae Kleb., is a globally prevalent disease affecting sunflower production. In this study, we identified a major quantitative trait locus (QTL) on chromosome 10 and other genomic regions associated with SVW resistance by integrating biparental and association mapping in sunflower populations from the National Institute of Agricultural Technology. Nine replicated field trials were conducted in highly infested V. dahliae reservoirs to assess disease incidence and severity. Both mapping populations were genotyped using double-digest restriction-site-associated DNA sequencing (ddRADseq). Association mapping with 18,161 SNPs and biparental QTL mapping with 1769 SNPs identified a major QTL on chromosome 10 explaining up to 30% of phenotypic variation for disease incidence at flowering and for the area under the disease progress curve for disease incidence, and which contributes to a lesser extent to disease severity reduction. Additional QTLs on chromosomes 17, 8, 9, 14, 13, and 11 were associated with reduced disease incidence, severity, or both. Candidate genes were identified within these associated regions, 39 of which are in the major QTL on Chromosome 10. These findings demonstrate the value of integrating complementary QTL mapping strategies for validating resistance loci and advancing sunflower breeding for SVW resistance.

Details

Title
Combining Linkage and Association Mapping Approaches to Study the Genetic Architecture of Verticillium Wilt Resistance in Sunflower
Author
Montecchia, Juan F 1   VIAFID ORCID Logo  ; Fass, Mónica I 2 ; Domínguez Matías 3   VIAFID ORCID Logo  ; González, Sergio A 2   VIAFID ORCID Logo  ; García, Martín N 2   VIAFID ORCID Logo  ; Filippi, Carla V 4 ; Ben Guerrero Emiliano 2 ; Maringolo Carla 5 ; Troglia Carolina 5   VIAFID ORCID Logo  ; Quiroz, Facundo J 5 ; González, Julio H 3   VIAFID ORCID Logo  ; Alvarez, Daniel 6   VIAFID ORCID Logo  ; Heinz, Ruth A 2 ; Lia, Verónica V 7   VIAFID ORCID Logo  ; Paniego, Norma B 2   VIAFID ORCID Logo 

 Instituto de Agrobiotecnología y Biología Molecular—IABIMO—INTA-CONICET, Instituto de Biotecnología, Centro de Investigaciones de Ciencias Veterinarias y Agronómicas, INTA, Hurlingham B1686, Argentina; [email protected] (M.I.F.); [email protected] (S.A.G.); [email protected] (M.N.G.); [email protected] (C.V.F.); [email protected] (E.B.G.); [email protected] (R.A.H.), Advanta Semillas S.A.I.C., Estación Experimental Venado Tuerto, Ruta Nac. 33 km 636, Venado Tuerto PC 2600, Argentina 
 Instituto de Agrobiotecnología y Biología Molecular—IABIMO—INTA-CONICET, Instituto de Biotecnología, Centro de Investigaciones de Ciencias Veterinarias y Agronómicas, INTA, Hurlingham B1686, Argentina; [email protected] (M.I.F.); [email protected] (S.A.G.); [email protected] (M.N.G.); [email protected] (C.V.F.); [email protected] (E.B.G.); [email protected] (R.A.H.) 
 Estación Experimental Agropecuaria Pergamino, INTA, Av. Frondizi km 4.5, Pergamino B2700, Argentina; [email protected] (M.D.); [email protected] (J.H.G.) 
 Instituto de Agrobiotecnología y Biología Molecular—IABIMO—INTA-CONICET, Instituto de Biotecnología, Centro de Investigaciones de Ciencias Veterinarias y Agronómicas, INTA, Hurlingham B1686, Argentina; [email protected] (M.I.F.); [email protected] (S.A.G.); [email protected] (M.N.G.); [email protected] (C.V.F.); [email protected] (E.B.G.); [email protected] (R.A.H.), Laboratorio de Bioquímica, Departamento de Biología Vegetal, Facultad de Agronomía, Universidad de la República, Avenida Garzón 780, Montevideo 12900, Uruguay 
 Estación Experimental Agropecuaria Balcarce, INTA, Ruta 226 km 73.5, Balcarce B7620, Argentina; [email protected] (C.M.); [email protected] (C.T.); [email protected] (F.J.Q.) 
 Estación Experimental Agropecuaria Manfredi, INTA, Ruta Nac. 9 km 636, Manfredi X5988, Argentina; [email protected] 
 Instituto de Agrobiotecnología y Biología Molecular—IABIMO—INTA-CONICET, Instituto de Biotecnología, Centro de Investigaciones de Ciencias Veterinarias y Agronómicas, INTA, Hurlingham B1686, Argentina; [email protected] (M.I.F.); [email protected] (S.A.G.); [email protected] (M.N.G.); [email protected] (C.V.F.); [email protected] (E.B.G.); [email protected] (R.A.H.), Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Intendente Güiraldes 2160, Ciudad Autónoma de Buenos Aires C1428, Argentina 
First page
1187
Publication year
2025
Publication date
2025
Publisher
MDPI AG
e-ISSN
22237747
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3194637218
Copyright
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.