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© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

Black spot is a major postharvest disease of cherry tomatoes, caused by Alternaria alternata. This causes economic losses and storage challenges, so researchers are exploring alternative methods. The biological control of fruits and vegetables using antagonistic bacteria and yeasts is currently a research hotspot. Initially, the biological control impact of Bacillus velezensis T3 on cherry tomato black spot was investigated. Disease defense, scavenging reactive oxygen species, and antioxidant-related enzymes were determined during different storage periods. The relative gene expressions of these enzymes were also confirmed using RT-qPCR. The results showed that B. velezensis T3 reduced the incidence of black spot disease in cherry tomatoes. The growth of A. alternata was suppressed by B. velezensis T3 cell-free filtrate both in vitro and in vivo. In addition, B. velezensis T3 induced the activities of disease resistance-related enzymes such as polyphenol oxidase (PPO), phenylalanine ammonia-lyase (PAL), β-1,3-glucanase (GLU), and chitinase (CHI), and the activities of the ROS-related enzymes superoxide dismutase (SOD), catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX), and reduced the rate of O2 production and H2O2, and MDA content of cherry tomatoes. This approach offers a promising alternative for extending shelf life, though further studies are needed to fully characterize its effects on fruit quality.

Details

Title
Biological Control of Black Spot Disease in Cherry Tomato Caused by Alternaria alternata with Bacillus velezensis T3
Author
Xinmeng, Wei 1 ; Yang Qiya 2 ; Dhanasekaran, Solairaj 2 ; Godana Esa Abiso 2 ; Zhang, Xi 2 ; Li, Yu 3 ; Liu, Xiaoyong 3 ; Zhang, Hongyin 2   VIAFID ORCID Logo 

 School of Life Sciences, Jiangsu University, Zhenjiang 212013, China; [email protected] 
 School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China; [email protected] (D.S.); [email protected] (E.A.G.); [email protected] (X.Z.) 
 College of Food Science and Technology, Henan Agricultural University, Zhengzhou 450002, China; [email protected] (Y.L.); [email protected] (X.L.) 
First page
1700
Publication year
2025
Publication date
2025
Publisher
MDPI AG
e-ISSN
23048158
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3211964249
Copyright
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.