Characterization and Antibacterial Activity Screening of Actinomycetes Isolated from Mangrove Sediment in Labuhan, Madura Island

Abstract

Actinomycetes are unique bacteria that form a branching network of hyphae. They are aerobic Gram-positive bacteria that are primarily found in soils and also have been reported that can be found in the estuary environments like mangrove sediment. These bacteria are known as the most prolific producers of microbial bioactive secondary metabolites, especially antibiotics. The purpose of this study was to isolate and determine the antibacterial activity of actinomycetes derived from mangrove sediment against Staphylococcus aureus and Escherichia coli. Sediment collected in the Labuhan Mangrove Education Park, Madura island. Isolation and characterization of actinomycetes was conducted by dilution plating on 2 different Actinomycetes media, such Starch Casein Agar (SCA) and ISP 2 (International Streptomycetes Project 2) medium. Identification of actinomycetes was performed based on morphological characters. A total of 12 isolates had been successfully isolated from SCA media and there were 13 isolates of actinomycetes bacteria successfully isolated from mangrove ISP 2 media. Five isolates from SCA medium have robust growth inhibition activity against S. aureus: A.S.14 (11.84mm± 0.39), A.S.13 (11.11± 0.04), A.S.7 (10.09 ± 1.45), A.S.15 (10,36 ± 1.13), and A.S.5 (11.88 ± 1.96), actinomycetes A.S.14 and A.S.13 were active against E. coli.