Abstract

Actinomycetes are unique bacteria that form a branching network of hyphae. They are aerobic Gram-positive bacteria that are primarily found in soils and also have been reported that can be found in the estuary environments like mangrove sediment. These bacteria are known as the most prolific producers of microbial bioactive secondary metabolites, especially antibiotics. The purpose of this study was to isolate and determine the antibacterial activity of actinomycetes derived from mangrove sediment against Staphylococcus aureus and Escherichia coli. Sediment collected in the Labuhan Mangrove Education Park, Madura island. Isolation and characterization of actinomycetes was conducted by dilution plating on 2 different Actinomycetes media, such Starch Casein Agar (SCA) and ISP 2 (International Streptomycetes Project 2) medium. Identification of actinomycetes was performed based on morphological characters. A total of 12 isolates had been successfully isolated from SCA media and there were 13 isolates of actinomycetes bacteria successfully isolated from mangrove ISP 2 media. Five isolates from SCA medium have robust growth inhibition activity against S. aureus: A.S.14 (11.84mm± 0.39), A.S.13 (11.11± 0.04), A.S.7 (10.09 ± 1.45), A.S.15 (10,36 ± 1.13), and A.S.5 (11.88 ± 1.96), actinomycetes A.S.14 and A.S.13 were active against E. coli.

Details

Title
Characterization and Antibacterial Activity Screening of Actinomycetes Isolated from Mangrove Sediment in Labuhan, Madura Island
Author
Kartika Dewi 1 ; Ilyas, Muhammad 1 ; Sabdaningsih, Aninditia 2 ; Arisandi, Apri 1 

 Department of Marine Sciences Faculty of Agriculture, Trunojoyo Madura University , Bangkalan 69162, Indonesia 
 Department of Aquatic Resources, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro , Semarang 50275, Indonesia 
First page
012021
Publication year
2025
Publication date
May 2025
Publisher
IOP Publishing
ISSN
17551307
e-ISSN
17551315
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3213466742
Copyright
Published under licence by IOP Publishing Ltd. This work is published under https://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.