Abstract

In insects, rapid degradation of odorants in antennae is extremely important for the sensitivity of olfactory receptor neurons. Odorant degradation in insect antennae is mediated by multiple enzymes, especially the carboxylesterases (CXEs) and glutathione S -transferases (GSTs). The Asiatic rice borer, Chilo suppressalis, is an economically important lepidopteran pest which causes great economic damage to cultivated rice crops in many Asian countries. In this study, we identified 19 putative CXE and 16 GST genes by analyzing previously constructed antennal transcriptomes of C. suppressalis . BLASTX best hit results showed that these genes are most homologous to their respective orthologs in other lepidopteran species. Phylogenetic analyses revealed that these CXE and GST genes were clustered into various clades. Reverse-transcription quantitative polymerase chain reaction assays showed that three CXE genes (CsupCXE8, CsupCXE13, and CsupCXE18) are antennae-enriched. These genes are candidates for involvement in odorant degradation. Unexpectedly, none of the GST genes were found to be antennae-specific. Our results pave the way for future researches of the odorant degradation mechanism of C. suppressalis at the molecular level.

Details

Title
Identification of Putative Carboxylesterase and Glutathione S -transferase Genes from the Antennae of the Chilo suppressalis (Lepidoptera: Pyralidae)
Author
Liu, Su; Zhong-Jun, Gong; Xiang-Jun, Rao; Mao-Ye, Li; Shi-Guang, Li
Publication year
2015
Publication date
2015
Publisher
Oxford University Press
e-ISSN
15362442
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3215571125
Copyright
© The Author 2015. Published by Oxford University Press on behalf of the Entomological Society of America. This work is published under http://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.