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© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

In line with the recommended European policy for a zero-waste crop supply chain, a lab-pilot optimisation process to valorise the by-products of industrially produced rapeseed meal (RM) was performed. Three batches of RM were first processed into ethanol-wash solutes (EWS) and then optimised (OEWS) by an ultrasound-assisted (UA) treatment. After direct analysis in real time–high resolution mass spectrometry (DART-HRMS) analysis, data were processed applying a partial least square–discriminant analysis (PLS-DA), which retrieved the 15 most discriminative ions able to characterise the biochemical changes during the ethanol-washing and UA optimisation process. The metabolomic fingerprinting of EWS and OEWS generated an accurate and well-defined 3D spatial clusterisation based on a restricted pool of informative bioactive compounds. A significantly higher relative abundance of sinapic, azelaic, and vernolic acids and a lower incidence of the oleic and palmitic fatty acids were detected in OEWS. DART-HRMS generated a vast amount of biochemical information in one single run, also demonstrating that its association with an untargeted multivariate statistical approach would be a valuable tool for revealing specific functional biomarkers. This would eventually enhance the circular and effective use of rapeseed residuals coming from this plant’s oilseed industry.

Details

Title
DART-HRMS for the Rapid Assessment of Bioactive Compounds in Ultrasound-Processed Rapeseed Meal By-Product
Author
Lante, Anna 1   VIAFID ORCID Logo  ; Massaro, Andrea 2   VIAFID ORCID Logo  ; Zacometti Carmela 2   VIAFID ORCID Logo  ; Mihaylova Dasha 3   VIAFID ORCID Logo  ; Vesela, Chalova 4   VIAFID ORCID Logo  ; Krastanov Albert 3   VIAFID ORCID Logo  ; Kalaydzhiev Hristo 5   VIAFID ORCID Logo  ; Cisneros Miluska 1   VIAFID ORCID Logo  ; Morbin Greta 2   VIAFID ORCID Logo  ; Riuzzi Giorgia 6 ; Segato Severino 6   VIAFID ORCID Logo  ; Tata, Alessandra 2   VIAFID ORCID Logo 

 Department of Agronomy, Food, Natural Resources, Animals, and Environment-DAFNAE, Padova University, Viale dell’Università, 16, 35020 Legnaro, Italy; [email protected] (A.L.); [email protected] (M.C.) 
 Experimental Chemistry Laboratory, Istituto Zooprofilattico Sperimentale delle Venezie, Viale Fiume, 78, 36100 Vicenza, Italy; [email protected] (A.M.); [email protected] (C.Z.); [email protected] (G.M.); [email protected] (A.T.) 
 Department of Microbiology and Biotechnology, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria; [email protected] (D.M.); [email protected] (A.K.) 
 Department of Biochemistry and Nutrition, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria; [email protected] 
 Department of Analytical Chemistry and Physical Chemistry, University of Food Technologies, 26 Maritza Blvd., 4002 Plovdiv, Bulgaria; [email protected] 
 Department of Animal Medicine, Production and Health, Padova University, Viale dell’Università, 16, 35020 Legnaro, Italy; [email protected] 
First page
5952
Publication year
2025
Publication date
2025
Publisher
MDPI AG
e-ISSN
20763417
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3217722661
Copyright
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.