SELECTION OF SOME ESSENTIAL OILS IN ORDER TO OBTAIN NEW INNOVATIVE ANTI-INFECTIVE MEDICINES

Abstract

The design of new molecules is a long process that requires massive financial investments, so the selection of essential oils known for their anti-infective effect and their association with antibiotics is an optimal solution. [...]the present study aimed to analyze the composition of essential oils as well as the structural analysis of conventional antibiotics in order to associate them with the aim of obtaining functional experimental models, with the potential to become anti-infective drugs. The concentrations of the bioactive constituents of the essential oils are within the limits of the European Pharmacopoeia 11" Ed. and the NMR and IR spectra confirmed the chemical structures of the conventional antibiotics. Combinations of essential oils associated with antibiotics showed stability especially when stored in a refrigerator (2-8 °C).

Objectives. Currently, the phenomenon of bacterial resistance has expanded significantly and therefore, the treatment of infectious diseases has become a real challenge. The design of new molecules is a long process that requires massive financial investments, so the selection of essential oils known for their anti-infective effect and their association with antibiotics is an optimal solution. Thus, the present study aimed to analyze the composition of essential oils as well as the structural analysis of conventional antibiotics in order to associate them with the aim of obtaining functional experimental models, with the potential to become anti-infective drugs.

Materials and methods. The research activity within the project was carried out on the following essential oils: Caryophylli aetheroleum, Origani aetheroleum, Eucalypti aetheroleum, Menthae aetheroleum, Lavandulae aetheroleum, their composition being analyzed with the help of gas chromatography coupled with mass spectrometry. Also, the conventional antibiotics (Neomycin sulphate, Bacitracin and Tetracycline hydrochloride) were analyzed from a structural point of view with the help of NMR and IR spectra. After analysis of all components, the essential oils were combined as follows: Caryophylli aetheroleum + Origani aetheroleum, Caryophylli aetheroleum + Eucalypti aetheroleum, Caryophylli aetheroleum + Menthae aetheroleum, Eucalypti aetheroleum + Menthae aetheroleum, Eucalypti aetheroleum + Lavandulae aetheroleum and each associated with antibiotics conventional. Stability over time of all associations was also tested.

Results and discussion. The concentrations of the bioactive constituents of the essential oils are within the limits of the European Pharmacopoeia 11" Ed. and the NMR and IR spectra confirmed the chemical structures of the conventional antibiotics. Combinations of essential oils associated with antibiotics showed stability especially when stored in a refrigerator (2-8 °C).

Conclusions. The experimental functional models obtained can represent the starting point for the launch of new anti-infective phytopharmaceutical products and the range completion of phytotherapeutic products from the portfolio of the Cantacuzino National Military Medical Institute for Research and Development.

Funding source: The Internal Research Plan of the Cantacuzino National Military Medical Institute for Research and Development.

Obiective. Ín prezent, fenomenul derezistentá bacterianá s-a extins semnificativ si, prinurmare, tratamentul bolilor infectioase a devenit o adeváratá provocare. Proiectarea unor noi molecule este un proces indelungat ce necesitá investitii financiare masive, astfel incät selectarea uleiurilor esentiale cunoscute pentru efectul antiinfectios si asocierea acestora cu antibiotice reprezintá o solutie optimá. Astfel, studiul de fatá a urmárit analizarea compozitiei uleiurilor esentiale precum si analizarea din punct de vedere structural a antibioticelor conventionale in vederea asocierii acestora cu scopul de a obtine modele experimentale functionale, cu potential de a deveni medicamente antiinfectioase.

Materiale si metode. Activitatea de cercetare din cadrul proiectuluia fost realizatä pe urmätoarele uleiuri esentiale: Caryophylli aetheroleum, Origani aetheroleum, Eucalypti aetheroleum, Menthae aetheroleum, Lavandulae aetheroleum, compozitia acestora fiind analizatá cu ajutorul cromatografiei de gaze cuplatá cu spectrometrie de masá. De asemenea, antibioticele conventionale (Neomycin sulphate, Bacitracin si Tetracicline hydrochloride) au fost analizate din punct de vedere structural cu ajutorul spectrelor RMN si IR. Dupá analiza tuturor componentelor, uleiurile esentiale au fost combinate dupa cum urmeazá: Caryophylli aetheroleum + Origani aetheroleum, Caryophylli aetheroleum + Eucalypti aetheroleum, Caryophylli aetheroleum + Menthae aetheroleum, Eucalypti aetheroleum + Menthae aetheroleum, Eucalypti aetheroleum + Lavandulae aetheroleum si asociate fiecare in parte cu antibioticele conventionale. Stabilitatea in timp a tuturor asocierilor a fost de asemenea testata.

Rezultate si discutii. Concentratiile constituentilor bioactivi ale uleiurilor esentiale sunt in limitele din Farmacopeea Europeaná Ed. 11 iar spectrele RMN si IR au confirmat structurile chimice ale antibioticelor conventionale. Combinatiile de uleiuri esentiale asociate cu antibiotice au manifestat stabilitate in special la depozitarea la frigider (2-8 °C).

Concluzii. Modelele experimentale functionale obtinute pot reprezenta punctul de plecare pentru lansarea de noi produse fitofarmaceutice antiinfectioase si completarea gamei de produse fitoterapeutice din portofoliul Institutului National de Cercetare-Dezvoltare Medico-Militará Cantacuzino.

Sursa de finantare: Planul Intern de cercetare al Institutului National de Cercetare-Dezvoltare Medico-Militará Cantacuzino.

Sidebar

*Corresponding author: Razvan Neagu, e-mail: [email protected]