Abstract

Emamectin benzoate (EMB) is one of the high-efficacious pesticide commonly used to control the ferocious economic crop pest S. exigua in the field. The results of S. exigua life table treated with three lethal doses of EMB (LC5, LC20 and LC50) indicated that three lethal doses of EMB could all inhibited the proliferation of S. exigua population effectively by long-lasting inhibit the survival of the S. exigua larvae and per female fecundity significantly (p < 0.05), and the inhibition effects of key life parameters were affected by EMB stress in a dose-dependent manner. 67,123 unigenes with an N50 value of 1941 bp were assembled by RNA-seq data analyses and 44,113 were expressed more than 12 samples. Furthermore, 3517, 3139 and 5135 differentially expressed genes were identified under different lethal doses of EMB treatment. Finally, 16 hub genes and three olfaction-related genes were identified by WGCNA analysis as biomarkers for the EMB response, and the cAMP-dependent proteins, P450 metabolic-related genes and ABC transporter genes might have been involved in response to EMB stress in S. exigua.

Details

Title
Transcriptome profile analysis reveals the emamectin benzoate-induced genes associated with olfaction and metabolic detoxification in Spodoptera exigua Hübner (Lepidoptera: noctuidae)
Author
Sun, Yang 1 ; Tao, Simin 2 ; Zhang, Wen 2 ; Jiang, Bin 2   VIAFID ORCID Logo  ; Han-Yang, Dai 3 ; Bao-Sheng, Liu 3 ; Yong-Jun, Zhang 4 ; Xiang-dong, Kong 5 ; Zhao, Jing 3 ; Li-Xin, Bai 3 

1  Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu, People’s Republic of China; Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, People’s Republic of China 
2  Key Laboratory for Conservation and Use of Important Biological Resources of Anhui Province, Anhui Provincial Key Laboratory of Molecular Enzymology and Mechanism of Major Diseases, College of Life Sciences, Anhui Normal University, Wuhu, People’s Republic of China 
3  Institute of Plant Protection, Jiangsu Academy of Agricultural Sciences, Nanjing, People’s Republic of China 
4  Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, People’s Republic of China 
5  Institute of Bioinformatics, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, People’s Republic of China 
Pages
340-357
Publication year
2022
Publication date
Jan 2022
Publisher
Taylor & Francis Ltd.
ISSN
2689-5293
e-ISSN
2689-5307
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1080/26895293.2022.2052190
ProQuest document ID
3222596031
Copyright
© 2022 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This work is licensed under the Creative Commons  Attribution – Non-Commercial License http://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.