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© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

To address the issue of aflatoxin contamination, which poses a significant threat to food safety and human health, we have conducted extensive research. We have isolated a strain of Myroides odoratimimus (3J2MO) from the soil that exhibited remarkable efficiency in degrading various aflatoxin types, including AFB1, AFB2, AFG1, AFG2, and AFM1. SDS-PAGE analysis confirmed the purity of the enzymes to be over 95%. Through fluorescence assays, we quantified the enzymatic activity, with an AFB1 degradation rate of 95% achieved at 37 °C and a pH of 8.0. Further analysis using HPLC-MS/MS identified the degradation intermediates, revealing the mechanisms of lactone ring cleavage and epoxy group hydrolysis. GO/COG/KEGG annotations provided insights into the functions of these enzymes, with peroxidase linked to reactive oxygen species (ROS) generation and helicase associated with ATP-dependent conformational changes. Helicase, on the other hand, hydrolyzes ATP, driving conformational changes in AFB1 and facilitating its breakdown into non-toxic metabolites. The potential industrial-scale application of this discovery could significantly mitigate aflatoxin-related economic losses while minimizing chemical residues in the food chain.

Details

Title
Study on the Degradation of Aflatoxin B1 by Myroides odoratimimus 3J2MO
Author
Wang, Xue 1   VIAFID ORCID Logo  ; Yao-Yao, Gao 2 ; Wang, Dun 1 ; Zhang, Qi 3 ; Hao-Ran, Wang 4 ; Ting-Ting, Zhang 4 ; Meng-Jie, Zhu 5 ; Dong, Jing 4 ; Dong, Ling 4 ; Peng, Feng 4 ; Xue-Hui, Tang 4 ; Pei-Wu, Li 6 

 Xiangyang Academy of Agricultural Sciences, Xiangyang 441057, China; [email protected] (X.W.); [email protected] (H.-R.W.); [email protected] (T.-T.Z.); [email protected] (P.F.); [email protected] (X.-H.T.), Hubei Hongshan Laboratory, Wuhan 430061, China, Oil Crops Research Institute, Chinese Academy of Agriculture Science, Wuhan 430062, China; [email protected], Key Laboratory for Biotoxin Detection, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China 
 Oil Crops Research Institute, Chinese Academy of Agriculture Science, Wuhan 430062, China; [email protected], Key Laboratory for Biotoxin Detection, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China 
 Hubei Hongshan Laboratory, Wuhan 430061, China, Oil Crops Research Institute, Chinese Academy of Agriculture Science, Wuhan 430062, China; [email protected], Key Laboratory for Biotoxin Detection, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430062, China 
 Xiangyang Academy of Agricultural Sciences, Xiangyang 441057, China; [email protected] (X.W.); [email protected] (H.-R.W.); [email protected] (T.-T.Z.); [email protected] (P.F.); [email protected] (X.-H.T.) 
 Xiangyang Academy of Agricultural Sciences, Xiangyang 441057, China; [email protected] (X.W.); [email protected] (H.-R.W.); [email protected] (T.-T.Z.); [email protected] (P.F.); [email protected] (X.-H.T.), Hubei Hongshan Laboratory, Wuhan 430061, China, Oil Crops Research Institute, Chinese Academy of Agriculture Science, Wuhan 430062, China; [email protected], Key Laboratory for Biotoxin Detection, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China, College of Food Science and Engineering, Wuhan Polytechnic University, Wuhan 430062, China 
 Hubei Hongshan Laboratory, Wuhan 430061, China, Oil Crops Research Institute, Chinese Academy of Agriculture Science, Wuhan 430062, China; [email protected], Key Laboratory for Biotoxin Detection, Ministry of Agriculture and Rural Affairs, Wuhan 430062, China 
First page
724
Publication year
2025
Publication date
2025
Publisher
MDPI AG
e-ISSN
20797737
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3223878166
Copyright
© 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.