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AL001 Overuse of analgesics can affect the fertility biomarker Anti-Müllerian hormone in females. A translational study
L. Carlsen
Rigshospitalet Glostrup, Neurology Department, Glostrup, Denmark
The Journal of Headache and Pain 2025, 26(Suppl 2):AL001
Objective: Over-the-counter analgesics (OTC) have been associated with disrupted male endocrinology, while effects on female endocrinology remains nearly unknown. The aim was to understand the effect of long-term analgesic exposure in females with medication overuse headache (MOH) on Anti-Müllerian Hormone (AMH), a surrogate measure of female fertility.
Methods: Using a translational approach, an observational prospective clinical study was conducted to determine AMH-levels in females with MOH, in combination with pre-clinical investigation of primary granulosa cells (GC) to understand the effects of analgesics on GC-function.
Results: We included 21 females (mean-age 30.0 years; SD (7.3)) for AMH-measurement. AMH increased by 21% from baseline (mean 20.1 pmol/L; SD (8.7)) after withdrawal of analgesics ((mean 24.3pmol/L; SD (12.0)); p=0.0023). Exposing primary GCs to analgesics (acetaminophen (100 and 200 µM, n = 9–10) and ibuprofen (150 and 200 µM, n = 12–13)) did not reduce AMH-levels. In contrast, de novo DNA synthesis in GCs (n=6) exposed to acetaminophen was reduced with 78% (p=0.0036) compared to controls, suggesting that cellular proliferation was restricted.
Conclusion: Frequent use of OTC was associated with repressed AMH-levels likely through disruption of GC proliferation. Further research is crucial to investigate a potential effect of analgesics on adult female reproductive endocrinology.
AL002 Sex differences in RAMP1/RAMP2 expression in the human middle meningeal artery match functional response to CGRP
T. de Vries, D. Schutter1, R. Dammers2, A. Vincent2, A. H. J. Danser1, A. Maassen van den Brink1
1Erasmus MC University Medical Center, Internal Medicine, Division of Pharmacology and Vascular Medicine, Rotterdam, Netherlands; 2Erasmus MC University Medical Center, Neurosurgery, Rotterdam, Netherlands
Correspondence: T. de Vries
The Journal of Headache and Pain 2025, 26(Suppl 2):AL002
Objective: CGRP induces vasodilation after binding to the CGRP receptor (CLR-RAMP1), but can activate the adrenomedullin receptor (CLR-RAMP2) as well. Previously, age-dependent sex differences were observed for CGRP-induced relaxation of human middle meningeal arteries1. In addition, RAMP1 and RAMP2 mRNA expression was highly variable between patients2. The current study aims to investigate whether RAMP1 and RAMP2...