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Abstract
2019年7月,在云南省文山壮族苗族自治州马关县古林箐省级自然保护区(22°45’16.36″ N,103°57’43.17″ E; 海拔1 160 m)内采集到无尾目(Anura)蛙科(Ranidae)蛙属(Rana)标本1号,雄性成体,头体长38.3 mm。根据形态特征比较发现,该蛙皮肤光滑,背部褐色,肩上方有“人”形疣粒,体背后部有小疣粒,体腹呈浅黄色,胸、腹部有深色云斑,指端略膨大且无沟,后肢细长,胫长超过体长之半,跗褶部位有小疣,趾端呈小吸盘状且腹侧具沟,趾间全蹼,四肢背面有深褐色横纹,与采集自越南林同省郎边高原(Langbian Plateau,Lam Dong,Vietnam,模式产地)的越南趾沟蛙(Rana johnsi)形态鉴定特征基本一致,仅体型大小稍有差异。基于16S rRNA 基因片段的系统发育分析表明,该标本与越南趾沟蛙的样本构成单支系,且遗传距离较小(p-distance为0.8%),确定该标本为云南省两栖纲(Amphibia)分布新纪录物种。该物种的发现增加了古林箐省级自然保护区的两栖动物物种多样性。
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