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Copyright © 2025 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.

Abstract

ABSTRACT

The gut microbiota is intricately associated with the onset and progression of colorectal cancer (CRC), leading to significant interest in developing prevention and treatment strategies that leverage gut microbiota. In this study, we collected 57 samples from 19 CRC patients, comprising cancerous tissue, paracancerous tissue, and normal mucosa. Utilizing metagenomic sequencing and bioinformatics analysis, we identified differences in the microbiomes and their functional characteristics across the various tissue types. The results indicated that species such as Alistipes putredinis were predominantly found in normal tissues, while Pseudomonas putida was enriched in paracancerous tissue, and Malassezia restricta was prevalent in cancerous tissues. Furthermore, the microbial functions exhibited variability among the different tissue types. Random forest analysis suggested that Moraxella osloensis may be implicated in the onset and progression of colorectal cancer. We also classified the patients into three subgroups based on the anatomical location of the colorectum: right-sided colon, left-sided colon, and rectum. The subgroup analysis revealed that the microbiota enriched in normal mucosa and paracancerous tissue varied across different anatomical sites. These findings not only elucidate the characteristics of the microbiomes in the normal mucosa, paracancerous tissue, and cancerous tissues of CRC patients, thereby providing new potential targets for clinical diagnosis and treatment, but also contribute to the existing microbiome data pertinent to CRC research.

IMPORTANCE

This study provides crucial insights into the relationship between gut microbiota and colorectal cancer (CRC) by analyzing microbial communities in different tissue types and anatomical locations of CRC patients. We identified distinct microbial signatures, such as Alistipes putredinis in normal tissues and Malassezia restricta in cancerous tissues, indicating location-specific microbiomes with unique functional attributes. These findings suggest potential new biomarkers or therapeutic targets for CRC. The observed microbiota variations among right-sided colon, left-sided colon, and rectum cancers underscore the heterogeneity of CRC, pointing toward more personalized treatment strategies. By enhancing our understanding of the microbiome’s role in CRC, this research paves the way for innovative diagnostic tools and targeted therapies tailored to individual patient profiles. This work is essential for advancing clinical approaches to CRC management.

Details

Title
The characteristics of tissue microbiota in different anatomical locations and different tissue types of the colorectum in patients with colorectal cancer
Author
Liu, Lei 1 ; Shi, Jianguo 1 ; Wang, Hui 1 ; Du Hansong 1 ; Yang, Jia 2 ; Wei, Kai 1 ; Zhou Zhuohui 1 ; Li, Moli 1 ; Huang, Shuai 2 ; Zhan Lifang 1 ; Li, Guolong 3 ; Lv Yongling 4 ; Shen Hexiao 4   VIAFID ORCID Logo  ; Cai, Wei 1   VIAFID ORCID Logo 

 Department of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology 12443 https://ror.org/00p991c53 , Wuhan , Hubei , China, Hubei Provincial Engineering Research Center of Intestinal Microecological Diagnostics, Therapeutics, and Clinical Translation 675140 , Wuhan , Hubei , China 
 Department of Gastrointestinal Surgery and Intestinal Microenvironment Treatment Center, The Central Hospital of Wuhan, Tongji Medical College, Huazhong University of Science and Technology 12443 https://ror.org/00p991c53 , Wuhan , Hubei , China 
 School of Life Sciences, Hubei University 12563 https://ror.org/03a60m280 , Wuhan , Hubei , China 
 Hubei Provincial Engineering Research Center of Intestinal Microecological Diagnostics, Therapeutics, and Clinical Translation 675140 , Wuhan , Hubei , China, School of Life Sciences, Hubei University 12563 https://ror.org/03a60m280 , Wuhan , Hubei , China 
University/institution
U.S. National Institutes of Health/National Library of Medicine
Publication year
2025
Publication date
2025
Publisher
American Society for Microbiology
e-ISSN
23795077
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
3225668436
Copyright
Copyright © 2025 Liu et al. This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.