Abstract

Abstract

Background: Illumina's second-generation sequencing platform is playing an increasingly prominent role in modern DNA and RNA sequencing efforts. However, rapid, simple, standardized and independent measures of run quality are currently lacking, as are tools to process sequences for use in downstream applications based on read-level quality data.

Results: We present SolexaQA, a user-friendly software package designed to generate detailed statistics and at-a-glance graphics of sequence data quality both quickly and in an automated fashion. This package contains associated software to trim sequences dynamically using the quality scores of bases within individual reads.

Conclusion: The SolexaQA package produces standardized outputs within minutes, thus facilitating ready comparison between flow cell lanes and machine runs, as well as providing immediate diagnostic information to guide the manipulation of sequence data for downstream analyses.

Details

Title
SolexaQA: At-a-glance quality assessment of Illumina second-generation sequencing data
Author
Cox, Murray P; Peterson, Daniel A; Biggs, Patrick J
Pages
485
Publication year
2010
Publication date
2010
Publisher
BioMed Central
e-ISSN
14712105
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
901868938
Copyright
© 2010 Cox et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.