Abstract

Abstract

Background: The 1.8-kb mRNA was reported as one of the oncogenesis-related genes of Marek's disease virus (MDV). In this study, the bacterial artificial chromosome (BAC) clone of a MDV field strain GX0101 was used as the platform to generate mutant MDV to examine the functional roles of 1.8-kb mRNA.

Results: Based on the BAC clone of GX0101, the 1.8-kb mRNA deletion mutant GX0101Δ(A+C) was constructed. The present experiments indicated that GX0101Δ(A+C) retained a low level of oncogenicity, and it showed a decreased replication capacity in vitro and in vivo when compared with its parent virus, GX0101. Further studies in vitro demonstrated that deletion of 1.8-kb mRNA significantly decreased the transcriptional activity of the bi-directional promoter between 1.8-kb mRNA and pp38 genes of MDV.

Conclusion: These results suggested that the 1.8-kb mRNA did not directly influence the oncogenesis but related to the replication ability of MDV.

Details

Title
Deletion of 1.8-kb mRNA of Marek's disease virus decreases its replication ability but not oncogenicity
Author
Sun, Aijun; Li, Yanpeng; Wang, Jingyan; Su, Shuai; Chen, Hongjun; Zhu, Hongfei; Ding, Jiabo; Cui, Zhizhong
Pages
294
Publication year
2010
Publication date
2010
Publisher
BioMed Central
ISSN
1743-422X
Source type
Scholarly Journal
Language of publication
English
DOI
https://doi.org/10.1186/1743-422X-7-294
ProQuest document ID
902294690
Copyright
© 2010 Sun et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.