Abstract

Inclusion body hepatitis caused by avian adenovirus with highly pathogenic strain lead to heavy mortality and

unusual lesion like hydropericardium in commercial broilers. Samples were collected from an outbreak in

12000 broiler flock from Andhra Pradesh with sudden high mortality initiated from 32 days of age. Liver was

enlarged and showed petechae. Hydropericardium was extensive. 10% liver suspension inoculated to SPF

chicks produced mortality with hydropericardium and showed presence of intranuclear inclusions in the

hepatocytes. Viral DNA extracted from infected liver processed for PCR produced an amplicon of 890 bp.

Nucleotide sequence analysis using blast programme of NCBI showed 99% identity with fowl adenovirus.

Details

Title
Isolation, identification and molecular characterization of Inclusion body hepatitis virus
Author
Gaba, Amit; Parmar, Hitesh; Pal, J K; Prajapati, K S
First page
414
Section
Original Research
Publication year
2010
Publication date
Sep 2010
Publisher
Veterinary World
ISSN
09728988
e-ISSN
22310916
Source type
Scholarly Journal
Language of publication
English
ProQuest document ID
909441266
Copyright
Copyright Veterinary World Sep 2010