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Web End = Rheumatol Int (2015) 35:14311434 DOI 10.1007/s00296-014-3210-0
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Absence of salivary CCL28 in primary Sjgrens syndrome
Gabriela HernandezMolina Amanda M. Burkhardt Guadalupe Lima Albert Zlotnik Jose L. Betanzos Susana Bahena Luis Llorente
Abstract CCL28 is a mucosa-associated epithelial-cell-produced chemokine involved in oral defense. We assessed the level of CCL28 in saliva of primary Sjgrens syndrome (pSS) patients in comparison with healthy controls and correlated it with IgA salivary levels. We included 30 non-smoker pSS patients and 30 non-smoker healthy controls paired by age (5 years). Saliva samples were col
lected during the morning and kept frozen at 86 C until
the analysis. Fifty microliters of saliva was diluted 3:1 with water and analyzed for CCL28 salivary levels by ELISA method. The samples were tested in triplicate. IgA salivary levels were tested by ELISA method. We used descriptive statistics, MannWhitney U test and Kendalls tau correlation coefcients. pSS patients were mostly females (93.3 %), mean age 54.5 13.3 years and median disease
duration of 7.6 years (0.533). Patients with pSS had lower levels of salivary CCL28 when compared with controls [0 (01,272 pg/ml) vs. 94.4 (05,810) pg/ml, p < 0.0001]. pSS patients also had lower median levels of salivary IgA [72.55 g/ml (0.40297.4)] than controls [131.9 g/ml (6.8281.8)], although the latter results did not reach statistical signicance (p = 0.51). Among the SS group, there
G. Hernandez-Molina G. Lima J. L. Betanzos L. Llorente (*)
Department of Immunology and Rheumatology, Instituto Nacional de Ciencias Mdicas y Nutricin Salvador Zubirn, Vasco de Quiroga 15. Tlalpan, 14000 Mexico City, D.F., Mexico e-mail: [email protected]
A. M. Burkhardt A. Zlotnik
Department of Physiology and Biophysics, School of Medicine, Institute for Immunology, University of California, Irvine, CA, USA
S. Bahena
Immunology Laboratory, S de LR de CV, Mexico City, Mexico
Received: 21 October 2014 / Accepted: 30 December 2014 / Published online: 8 January 2015 Springer-Verlag Berlin Heidelberg 2015
was no correlation between CCL28 and IgA salivary levels nor between salivary IgA and disease duration, salivary ow, serum...