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Benzocaine as "a convenient killing agent for amphibians" was first reported by Altig (1980) and then described by McDiarmid (1994). Altig placed a small dose of benzocaine-containing gel (e.g., Orajel(R) regular, 7.5% benzocaine) on a frog's head to kill it. Orajel(R) is a product that can be obtained easily at any pharmacy. We modified this technique by gently rubbing benzocaine (Orajel(R) regular, 7.5% benzocaine or Orajel(R) extra strength, 20% benzocaine) on the animal's ventral surface instead of its head. For anurans, benzocaine can be applied to the pelvic patch area rather than the entire venter. Benzocaine applied to the venter was absorbed faster than when applied to the head.
Hyla cinerea individuals were euthanized using both head and pelvic...