Alzheimer's disease risk genes in wild-type adult zebrafish exhibit gender-specific expression changes during aging

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Web End = Neurogenetics (2016) 17:197199 DOI 10.1007/s10048-016-0485-1

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Web End = LETTER TO EDITOR

Alzheimers disease risk genes in wild-type adult zebrafish exhibit gender-specific expression changes during aging

Jinyoung Lee1 & Samuel M. Peterson1 & Jennifer L. Freeman1

Received: 9 March 2016 /Accepted: 14 May 2016 /Published online: 28 May 2016 # Springer-Verlag Berlin Heidelberg 2016

To the editor:Alzheimers disease (AD) is a neurodegenerative disease and the most common type of dementia. It is recognized that certain genetic factors involved in formation of amyloid plaques (an AD pathological hallmark) including amyloid- protein precursor (APP, zebrafish appa and appb) and presenilins (zebrafish psen1 and psen2), and also apolipoprotein E (APOE, zebrafish apoea and apoeb) play a role in elevating the risk for AD [1]. However, the pathogenic mechanisms related with the presence of these AD genes are not yet clearly identified. The zebrafish model is a promising tool for investigating pathogenesis of neurodegenerative diseases including AD with high genetic similarity (~70 %) between humans and zebrafish [13]. However, wild-type adult zebrafish have not been widely applied in AD research due in part to limited characterization of disease risk genes. Therefore, it is necessary to investigate AD-risk genes in wild-type adult zebrafish for further application of this model to study AD pathogenesis.

AD risk is influenced by age and gender [4, 5]. To uncover the relationship of both age and gender, we investigated spatial and quantitative expression of AD-risk genes in adult zebrafish (AB strain) at two ages by gender. Spatial expression of appa, appb, psen1, and apoea genes was localized with in situ hybridization on brain sections of adult male and female zebrafish aged 3 and 12 months (Fig. 1a). Spatial localization was not found to be different between gender and age groups. The expression of appa, appb, psen1, and apoea was relatively ubiquitous in the adult zebrafish brain with scattered expression of the genes present throughout the brain. Moderate to extensive expression...