Content area
Full Text
Appl Biochem Biotechnol (2010) 160:856867
DOI 10.1007/s12010-009-8534-y
Received: 28 October 2008 /Accepted: 20 January 2009 /
Published online: 18 February 2009# Humana Press 2009
Abstract Polyvinylferrocene (PVF) was used as a mediator for the fabrication of a horseradish peroxidase (HRP)-modified electrode to detect phenol derivatives via a composite polymeric matrix of conducting polypyrrole (PPy). Through an electropolymerization process, enzyme HRP was entrapped with PPy in a three-electrode system onto a glassy carbon electrode previously covered with PVF, resulting in a composite polymeric matrix. Steady-state amperometric measurements were performed at 200 mV vs. Ag/AgCl in aqueous phosphate buffer containing NaCl 0.1 M (pH 6.8) in the presence of hydrogen peroxide. The response of the HRP-modified PVF electrode was investigated for various phenol derivatives, which were 4-chlorophenol, phenol, catechol, hydroquinone, 2-aminophenol, pyrogallol, m-cresol, and 4-methoxyphenol. Analytical parameters for the fabricated PVF electrode were obtained from the calibration curves. The highest sensitivity was obtained from the calibration of 4-chlorophenol as 29.91 nA/M. The lowest detection limit was found to be 0.22 M (S/N=3) for catechol, and the highest detection limit was found to be 0.79 M (S/N=3) for 4-methoxyphenol among the tested derivatives. The biosensor can reach 95% of steady-state current in about 5 min. The electrode is stable for 2 months at 4 C.
Keywords Phenol biosensor . Conducting polymer . HRP. PVF. Polypyrrole
Introduction
The importance of using biosensors for environmental surveillance becomes more prevalent in literature with the emphasis to phenol determination and control [1]. The accurate determination of phenols is of immense importance in environmental analysis, and the detrimental effect of phenols on human health requires a strict mandate for the
M. Topcu Sulak : E. Erhan : B. Keskinler (*)
Department of Environmental Engineering, Gebze Institute of Technology, 41400 Gebze, Turkey e-mail: [email protected]
Amperometric Phenol Biosensor Based on Horseradish Peroxidase Entrapped PVF and PPy Composite Film Coated GC Electrode
Meral Topcu Sulak & Elif Erhan & Blent Keskinler
Appl Biochem Biotechnol (2010) 160:856867 857
quantification of such compounds. The concentration of these compounds in natural water or soil may vary to some degree, but on the whole, they are present at the parts per billion level. A number of phenol compounds are listed in the European Community (EC) Directive 76/464/EEC concerning dangerous substances...