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Antonie van Leeuwenhoek (2008) 94:377387 DOI 10.1007/s10482-008-9254-y
ORIGINAL PAPER
Analysis of Salmonella Agona and Salmonella Weltevreden in Malaysia by PCR ngerprinting and antibiotic resistance proling
Lee Learn-Han Cheah Yoke-Kqueen Noorzaleha Awang Salleh Sabrina Sukardi Sim Jiun-Horng Khoo Chai-Hoon Son Radu
Received: 11 October 2007 / Accepted: 14 May 2008 / Published online: 12 June 2008 Springer Science+Business Media B.V. 2008
Abstract Forty-eight strains of Salmonella enterica subsp. enterica serovar Agona and 33 strains of Salmonella enterica subsp. enterica serovar Weltevreden were characterized by random amplied polymorphic DNA (RAPD) ngerprinting using 3 different arbitrary primer, Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR) and antimicrobial susceptibility testing. By using RAPD, 81 strains (44 strains of S. Agona and 33 strains of S. Weltevreden) can be clustered into 14 groups and 6 single isolates whereas ERIC-PCR produced 7 clusters and 3 single isolates. Thirteen antimicrobial agents were used and all the isolates were resistant to erythromycin and showed Multiple Antimicrobial Resistance indexes, ranging from 0.08 to 0.62. Poultry still remain as the common reservoir for multi-drug-resistant Salmonella. On the
other hand, vegetables contaminated with S. Weltevreden showed a gain in antimicrobial resistance. Besides that, consistent antibiograms were observed from S. Weltevreden isolated at Kajang wet market on 2000/08/02.
Keywords Salmonella Agona Salmonella
Weltevreden ERIC-PCR RAPD Multiple
antimicrobial resistance Composite analysis
Introduction
Salmonella spp. Enterobacteriaceae consist of more than 2400 serovars (Popoff et al. 2000) that causes food-borne salmonellosis, one of the most widespread food-borne diseases worldwide. In USA, the Centers for Disease Control and Prevention (CDC) have estimated that salmonellosis is responsible for 1.4 million illnesses annually (Mead et al. 1999). In Malaysia, Salmonella enterica subsp. enterica serovar Agona (S. Agona) and Salmonella enterica subsp. enterica serovar Weltevreden (S. Weltevreden) were the second and third most prevalent Salmonella serovars isolated from animal and livestock products over a ve-year period 19962001 after Salmonella Enteriditis (Maria et al. 2002).
Salmonella Agona seem to play a special role in food-borne diseases as they are one of the most commonly isolated Salmonella enterica in U.S pigs market (Bahnson et al. 2006). This serovar represents
L. Learn-Han C. Yoke-Kqueen (&)
S. Sukardi S. Jiun-Horng K. Chai-Hoon
Department of Biomedical Science, Faculty of Medicine and Health Sciences, University Putra Malaysia, UPM Serdang, Selangor Darul...