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Eur J Nutr (2012) 51:2937 DOI 10.1007/s00394-011-0186-3
ORIGINAL CONTRIBUTION
Anti-oxidant, anti-inammatory and immunomodulating properties of an enzymatic protein hydrolysatefrom yellow eld pea seeds
Fatou Ndiaye Tri Vuong Jairo Duarte
Rotimi E. Aluko Chantal Matar
Received: 20 November 2010 / Accepted: 14 March 2011 / Published online: 27 March 2011 Springer-Verlag 2011
AbstractPurpose Enzymatic protein hydrolysates of yellow pea seed have been shown to possess high anti-oxidant and anti-bacterial activities. The aim of this work was to conrm the anti-oxidant, anti-inammatory and immunomodulating activities of an enzymatic protein hydrolysate of yellow eld pea seeds.
Methods The anti-oxidant and anti-inammatory properties of peptides from yellow eld pea proteins (Pisum sativum L.) were investigated in LPS/IFN-c-activated
RAW 264.7 NO(-) macrophages. The immunomodulating potential of pea protein hydrolysate (PPH) was then studied in a murine model.
Results Pea protein hydrolysate, after a 12 h pre-treatment, showed signicant inhibition of NO production by activated macrophages up to 20%. Moreover, PPH significantly inhibited their secretion of pro-inammatory cytokines, TNF-a- and IL-6, up to 35 and 80%, respectively.
Oral administration of PPH in mice enhanced the
phagocytic activity of their peritoneal macrophages and stimulated the gut mucosa immune response. The number of IgA? cells was elevated in the small intestine lamina propria, accompanied by an increase in the number of IL-4?, IL-10? and IFN-c? cells. This was correlated to up-regulation of IL-6 secretion by small intestine epithelial cells (IEC), probably responsible for B-cell terminal differentiation to IgA-secreting cells. Moreover, PPH might have increased IL-6 production in IECs via the stimulation of toll-like receptors (TLRs) family, especially TLR2 and TLR4 since either anti-TLR2 or anti-TLR4 was able to completely abolish PPH-induced IL-6 secretion. Conclusions Enzymatic protein degradation confers anti-oxidant, anti-inammatory and immunomodulating potentials to pea proteins, and the resulted peptides could be used as an alternative therapy for the prevention of inammatory-related diseases.
Keywords Anti-oxidant Anti-inammatory
Immunomodulating Pea proteins Cytokines
Introduction
A growing body of evidence has shown that peptides from milk and soy proteins can prevent cancer due to their anti-oxidant and anti-inammatory effects [1, 2]. Pea proteins are of great nutritional importance being one of the major food legumes grown in different parts of the world [3]. Therefore, enzymatic digestion of pea proteins may bring some improvement in the matter of...