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Abstract
Early childhood caries (ECC) is one of the major oral health issues in children aged <71 months owing to multifactorial causes, including accumulation of Streptococcus mutans biofilm. This study I aimed to analyze the effectiveness of virgin coconut oil (VCO) mousse at different concentrations I against S. mutans biofilm in children with ECC.
Cell viability was analyzed to estimate biofilm growth on VCO at different concentrations (0.8%, 8%, and 80%) by crystal violet assay in 96-microwell plates and by measuring colony-forming units. I S. mutans colonies were counted on brain heart infusion agar plates. Inter-group trials were I analyzed by one-way analysis of variance (P<.05). The concentration of 80% VCO mousse showed I significant results compared with that of VCO mousse at 0.8% and 8% concentrations, showing I equal results obtained with the positive control which is casein phosphopeptide-amorphus calcium I phosphate (CPP-ACP). VCO mousse (80%) is an effective antibacterial agent against S. mutans in I children with ECC.
Experimental article (J Int Dent Med Res 2019; 12(2):429-433)
Keywords: Dental caries, Streptococcus mutans, Coconut oil.
Received date: 11 February 2019 Accept date: 17 April 2019
Introduction
Early childhood caries (ECC) is one of the major oral health issues that commonly affects infants and pre-school children worldwide. Especially among children living in a socially disadvantaged environment, it has become a significant problem. ECC is defined as the presence of one or more decayed, missing, or filled tooth surfaces in any of the primary teeth in a child aged <71 months.1 In children aged <3 years, presence of caries on the smooth surface of teeth is indicative of severe early childhood caries (S-ECC).2 Caries remains one of the most challenging health problems for children and adults in Indonesia. According to a study in Jakarta, the prevalence of caries in children aged 3-5 years is 81.2%, with a national DMFT index of 4.6 in 2013.3
Streptococcus mutans is a gram-positive facultative anaerobic bacteria that is associated with dental caries.4 It plays a very important role in the etiology of caries, with its ability to attach to the enamel salivary pellicle and to other plaque bacteria. It is a strong acid producer that can create an acidic environment, increasing the risk of cavities. S. mutans possesses a...