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Abstract
Background:
ESBL producing bacteria poses a major problem for clinical therapautics. The ESBL producing isolates of E.coli among other clinical isolates has been increasing over the past few years resulting in limitation of therapeutic options.
Materials and methods:
A total number of 20 clinical specimens of E.coli were obtained from different clinical samples. They were subjected for the antibiotic susceptibility pattern by Kirby bauer disc diffusion method. The 3rd and 4th generation cephalosporins resistant isolates will be detected for ESBL production.
Results:
In our isolates, we have found increased percentage 14/20 (70%) of isolates showed sensitivity to amikacin followed by gentamicin, which showed sensitivity of 9/20 (45%). 80-90% of E.coli isolates showed resistance to cephalosporin group of drugs. All E. coli isolates 20/20 (100%) were found to be extended spectrum beta lactamase producers using Ceftazidime (30µg), ceftazidime/clavulanic acid (30/10µg) and 11/20 (55%) of isolates were shown to be positive for ESBL using cefotaxime (30µg), cefotaxime/clavulanic acid (30/10µg).
Conclusion:
The study reveals higher percentage of isolates were resistant to different drugs and ESBL producers were more. We conclude that resistant to cephalosporins were due to extended spectrum beta lactamase production in our isolates.
Keywords: E.coli, antibiotic sensitivity testing, ESBL
INTRODUCTION
Urinary tract infection is one of the most important causes of morbidity and mortality.[1] Escherichia coli is the most common urinary pathogen isolated from 50-90% of all the uncomplicated urinary tract infections.[2]
Extended spectrum beta lactamases (ESBL) were documented globally. [3] The advent of ESBL producers as posed a great problem to the use of many antibiotics particularly cephalosporin. [4]
MATERIALS AND METHODS
Bacterial isolates
A total of 20 non repetitive clinical isolates of E.coli were collected from Saveetha Medical College, Chennai. They were processed for a battery of standard biochemical tests and confirmed. Isolates were preserved in semisolid Trypticase soy broth stock and stored at 4°C until further use.
Antibiotic susceptibility testing
Antibiotic susceptibility test was determined for these isolates to routinely used antibiotics such ampicillin, amoxicillin, ,amikacin ,norfloxacin, ceftazidime, cefotaxime, ciprofloxacin and gentamicin as by Kirby Bauer disc diffusion method. [5]
Detection of ESBL in E. coli
E.coli producing ESBL were detected by disc potentiation test. Overnight culture suspensions of E.coli isolates were adjusted...