Antioxidant Activity, Total Phenolic and Flavonoid Contents of Some Tanacetum L. (Asteraceae) Taxa Growing in Turkey/Türkiye'de Yetisen Bazi Tanacetum L. (Asteraceae) Taksonlarinin Antioksidan Aktivitesi, Total Fenolik ve Flavonoit Içerikleri

Headnote

SUMMARY

In this study, five Tanacetum taxa (T. armenum (DC.) Schultz Bip., T. cadmeum (Boiss.) Heywood ssp. cadmeum, T cilicicum (Boiss.) Grierson, T. praeteritum (Horw.) Heywood ssp. massicyticum Heywood, T. praeteritum (Horw.) Heywood ssp. praeteritum) collected from Antalya were investigated for their possible in vitro antioxidant activity. For this purpose, methanol extracts of the aerial parts of these plants were tested with four complementary methods, namely 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity, 2,2,'azinobis (3'ethylbenzothiazolin'6' sulphonic acid) (ABTS) radical cation scavenging capacity, cupric ion reducing antioxidant capacity (CUPRAC) and ferric-reducing antioxidant power (FRAP) assays. Moreover, total phenolic contents of plant extracts were determined by Folin-Ciocalteus reagent and their flavonoid contents were determined by aluminum chloride colorimetric method. Results of present study revealed that, all plant extracts exhibited antioxidant effect with various potencies. Also it was determined that, T. praeteritum ssp. massicyticum, which showed the highest scavenging activity against DPPH radical (IC5=197.82 pg/mL) and ABTS radical cation (191.41 mg Trolox equivalents/g extract) as well as possessed the highest cupric ion reducing antioxidant capacity (138.86 mg gallic acid equivalents/g extract) among the plants studied, had also the highest total phenolic (149.93 mg gallic acid equivalents/g extract) and flavonoid (33.42 mg quercetin equivalents/g extract) contents. Furthermore, T. cilicicum which showed lower activity than other plant extracts on DPPH, ABTS and CUPRAC assays, was determined to possess the highest ferric reducing antioxidant power (198.19 mg quercetin equivalents/g extract).

Key Words: Tanacetum, Asteraceae, Antioxidant activity, Total phenolic content, Total flavonoid content, Antalya.

Türkiye'de Yetisen Bazi Tanacetum L. (Asteraceae) Taksonlarinin Antioksidan Aktivitesi, Total Fenolik ve Flavonoit Içerikleri

ÖZET

Bu çaliçmada, Antalya ilinden toplanan be§ Tanacetum taksonunun (T armenum (DC.) Schultz Bip., T. cadmeum (Boiss.) Hey wood ssp. cadmeum, T cilicicum (Boiss.) Grierson, T praeteritum (Horw.) Heywood ssp. massicyticum Heywood, T. praeteritum (Horw.) Heywood ssp. praeteritum) olasi in vitro antioksidan aktivitesi ara§tinlmi§tir. Bu amaçla, bitkilerin toprak üstü kisimlarindan hazirlanan metanol ekstreleri, 2,2-difenil-1-pikrilhidrazil (DPPH) radikal süpürücü kapasite tayini, 2,2>-azinobis (3-etilbenzotiyazolin'6'sülfonik asit) (ABTS) radikalkatyonusüpürücü aktivite tayini, bakir iyonu indirgeyici antioksidan kapasite (CUPRAC) tayini ve demir iyonu indirgeyici antioksidan güç (FRAP) tayini olmak üzere birbirini tamamlayici dört farkli yöntem ile test edilmipir. Ayrica, ekstrelerin içerigindeki total fenolik madde miktarlari Folin Ciocalteu reaktifi kullanilarak, total flavonoit miktarlari ise alüminyum klorür kolorimetrik yöntemiyle tespit edilmipir. Çali^mamizin sonuçlari,...