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Mol Biol Rep (2014) 41:29172921 DOI 10.1007/s11033-014-3147-0
Association of ADIPOQ gene with obesity and adiponectin levels in Malaysian Malays
Yamunah Devi Apalasamy Sanjay Rampal
Agus Salim Foong Ming Moy Awang Bulgiba
Zahurin Mohamed
Received: 6 September 2012 / Accepted: 13 January 2014 / Published online: 22 January 2014 Springer Science+Business Media Dordrecht 2014
Abstract Studies have shown that single-nucleotide polymorphisms (SNPs) on the ADIPOQ gene have been linked with obesity and with adiponectin levels in various populations. Here, we aimed to investigate the association of ADIPOQ rs17366568 and rs3774261 SNPs with obesity and with adiponectin levels in Malaysian Malays. Obesity parameters and adiponectin levels were measured in 574 subjects. Genotyping was performed using real-time polymerase chain reaction and Sequenom MassARRAY. A signicant genotypic association was observed between ADIPOQ rs17366568 and obesity. The frequencies of AG and AA genotypes were signicantly higher in the obese group (11 %) than in the non-obese group (5 %) (P = 0.024). The odds of A alleles occurring among the obese group were twice those among the non-obese group (odds ratio 2.15; 95 % condence interval 1.134.09). However, no signicant association was found between allelic frequencies of ADIPOQ rs17366568 and obesity after Bonferroni correction (P [ 0.025) or between ADI
POQ rs3774261 and obesity both at allelic and genotypic levels. ADIPOQ SNPs were not signicantly associated with log-adiponectin levels. GA, GG, and AG haplotypes
of the ADIPOQ gene were not associated with obesity. We conrmed the previously reported association of ADIPOQ rs17366568 with the risk of obesity. ADIPOQ SNPs are not important modulators of adiponectin levels in this population.
Keywords Body mass index Adiponectin variants
Polymorphism Haplotypes
Introduction
Adiponectin is the most abundant protein secreted by adi-pose tissues [1]. Its circulating level is inversely correlated with body mass index (BMI) [2]. Contrary to those of most other adipokines, adiponectin levels are decreased in obesity and increased upon weight reduction [3]. Previous studies have shown that adiponectin is associated with energy homeostasis, glucose, and lipid metabolism [4]. Low adiponectin levels may lead to increased risks of obesity, insulin resistance, diabetes, and cardiovascular disease [5]. Adiponectin has been identied as a potential target for therapeutic intervention in obesity, diabetes, and other cardiovascular disorders [6].
Serum adiponectin levels are highly heritable and associated...